TY - JOUR
T1 - A methodological approach based on gold-nanoparticles followed by matrix assisted laser desorption ionization time of flight mass spectrometry for the analysis of urine profiling of knee osteoarthritis
AU - López-Cortés, Rubén
AU - Formigo, Jacobo
AU - Reboiro-Jato, Miguel
AU - Fdez-Riverola, Florentino
AU - Blanco, Francisco J.
AU - Lodeiro, Carlos
AU - Oliveira, Elisabete
AU - Capelo, J. L.
AU - Santos, Helena M.
N1 - Sem PDF.
Fundacao para a Ciencia e a Tecnologia, Ministerio da Ciencia, Tecnologia e Ensino Superior (FCT, Portugal) (SFRH/BPD/72557/2010;SRFH/BPD/73997/2010)
Xunta de Galicia (Spain) (09CSA043383PR;10CSA383009PR)
PROTEOMASS Scientific Society (Portugal)
Fondo de Investigacion (PI-12/0329)
Maphre Foundation
PY - 2016/4/1
Y1 - 2016/4/1
N2 - The aim of this work is to develop a nanoparticle-based methodology to find out biomarkers of diagnostic for knee osteoarthritis, KOA, through the use of matrix assisted laser desorption ionization time-of-flight-based mass spectrometry profiling. Urine samples used for this study were obtained from KOA patients (42 patients), patients with prosthesis (58 patients), and controls (36 individuals) with no history of joint disease. Gold-nano particle MALDI-based urine profiling was optimized and then applied over the 136 individuals. Jaccard index and 10 different classifiers over MALDI MS datasets were used to find out potential biomarkers. Then, the specificity and sensitivity of the method were evaluated. The presence of ten m/z signals as potential biomarkers in the healthy versus non-healthy approach suggests that patients (KOA and prosthesis) are differentiable from the healthy volunteers through profiling. The automatic diagnostic study confirmed these preliminary conclusions. The sensitivity and the specificity for the urine profiling criteria here reported, achieved by the C4.5 classifier, is 97% and 69% respectively. Thus, it is confirmed the utility of the method proposed in this work as an additional fast, non-expensive and robust test for KOA diagnostic. When the proposed method is compared with those used in common practice it is found that sensitivity is the highest, thus with a low false negative rate for diagnostic KOA patients in the population studied. Specificity is lower but in the range accepted for diagnostic objectives.
AB - The aim of this work is to develop a nanoparticle-based methodology to find out biomarkers of diagnostic for knee osteoarthritis, KOA, through the use of matrix assisted laser desorption ionization time-of-flight-based mass spectrometry profiling. Urine samples used for this study were obtained from KOA patients (42 patients), patients with prosthesis (58 patients), and controls (36 individuals) with no history of joint disease. Gold-nano particle MALDI-based urine profiling was optimized and then applied over the 136 individuals. Jaccard index and 10 different classifiers over MALDI MS datasets were used to find out potential biomarkers. Then, the specificity and sensitivity of the method were evaluated. The presence of ten m/z signals as potential biomarkers in the healthy versus non-healthy approach suggests that patients (KOA and prosthesis) are differentiable from the healthy volunteers through profiling. The automatic diagnostic study confirmed these preliminary conclusions. The sensitivity and the specificity for the urine profiling criteria here reported, achieved by the C4.5 classifier, is 97% and 69% respectively. Thus, it is confirmed the utility of the method proposed in this work as an additional fast, non-expensive and robust test for KOA diagnostic. When the proposed method is compared with those used in common practice it is found that sensitivity is the highest, thus with a low false negative rate for diagnostic KOA patients in the population studied. Specificity is lower but in the range accepted for diagnostic objectives.
KW - Diagnostic
KW - Gold nanoparticle
KW - Knee osteoarthritis
KW - MALDI
KW - Profiling
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=84953722559&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2015.06.043
DO - 10.1016/j.talanta.2015.06.043
M3 - Article
C2 - 26838453
AN - SCOPUS:84953722559
VL - 150
SP - 638
EP - 645
JO - Talanta
JF - Talanta
SN - 0039-9140
ER -