@article{5da70e1121d1423199430bd517415e35,
title = "A mesofluidic platform integrating on-chip probe ultrasonication for multiple sample pretreatment involving denaturation, reduction, and digestion in protein identification assays by mass spectrometry",
abstract = "The integration of ultrasound (US)-assisted sample processing on-chip in a lab-on-a-valve (LOV) format for automated high-throughput shotgun proteomic assays is herein presented for the first time. The proof of concept of this system was demonstrated with the analysis of three proteins and sera from patients with lymphoma or myeloma.",
keywords = "A-VALVE, MICROFLUIDIC DEVICES, INFRARED RADIATION, FUTURE-PROSPECTS, ONLINE DIGESTION, PEPTIDE, SYSTEMS, FINGERPRINT, PROTEOMICS, TRYPSIN",
author = "Nunes-Miranda, {J{\'u}lio D.} and Cristina Nunez and Santos, {Hugo M.} and Gon{\c c}alo Vale and Miguel Reboiro-Jato and Florentino Fdez-Riverola and Carlos Lodeiro and Manuel Miro and Capelo, {Jos{\'e} Luis}",
note = "J. D. Nunes-Miranda acknowledges the doctoral grant SRFH/BD/80496/2011. C. Nunez thanks Xunta de Galicia for her postdoctoral contract (12C program). G. Vale and H. M. Santos, acknowledge the post-doctoral grants SFRH/BPD/73117/2010 and SRFH/BPD/73997/2010, respectively, provided by the Portuguese Foundation of Science and Technology (Fundacao para a Ciencia e a Tecnologia-Ministerio da Educacao e Ciencia). Manuel Miro acknowledges. financial support from the Spanish Ministry of Economy and competitiveness through project CTM2010-17214. The authors are grateful to Dr V. Cerda for the loan of analytical instrumentation. Scientific Society ProteoMass is acknowledged for financial support. The authors also thank REQUIMTE-FCT PEst-C/EQB/LA0006/2013.",
year = "2014",
doi = "10.1039/c3an02178e",
language = "English",
volume = "139",
pages = "992--995",
journal = "Analyst",
issn = "0003-2654",
publisher = "RSC - Royal Society of Chemistry",
number = "5",
}