TY - JOUR
T1 - A magnetic controllable tool for the selective enrichment of dimethoate from olive oil samples: a responsive molecular imprinting-based approach
AU - Garcia, Raquel
AU - Carreiro, Elisabete P
AU - Prates Ramalho, João P
AU - Mirão, J
AU - Burke, Anthony J
AU - Gomes da Silva, Marco D R
AU - Freitas, Ana Maria Costa
AU - Cabrita, Maria João
N1 - sem pdf conforme despacho.
Operation ALENT-07–0262-FEDER-001871/Laboratório de Biotecnologia Aplicada e Tecnologias Agro-Ambientais and FEDER Funds through the Operational Programme for Competitiveness Factors-COMPETE and National Funds through FCT – Foundation for Science and Technology under the Project UID/AGR/00115/2016 and the Project UID/QUI/0619/2016).
PY - 2018/7/15
Y1 - 2018/7/15
N2 - Aiming to develop a straightforward magnetic-based sample preparation methodology for the selective extraction of dimethoate from olive oil, the synthesis of dimethoate-imprinted polymer on the surface of modified magnetic nanoparticles has been attempted. Molecular recognition assays have proven their suitability for the selective pre-concentration of dimethoate. Mechanistic basis for template selective recognition has been explored using a quantum chemical approach, providing new insights about the mechanisms underlying template recognition. Thus, a magnetic molecularly imprinted solid-phase extraction method was developed allowing the extraction of dimethoate from spiked olive oil samples, at levels similar to the maximum residue limits imposed by legislation, followed by the quantification of their levels by high-performance liquid chromatography with diode-array detection. Recoveries of 94.55% were obtained, with relative standard deviations lower than 0.53% (n = 3). The developed sample preparation technique enables a selective pre-concentration/enrichment of dimethoate from olive oil matrix with minimum handling and less solvent consumption.
AB - Aiming to develop a straightforward magnetic-based sample preparation methodology for the selective extraction of dimethoate from olive oil, the synthesis of dimethoate-imprinted polymer on the surface of modified magnetic nanoparticles has been attempted. Molecular recognition assays have proven their suitability for the selective pre-concentration of dimethoate. Mechanistic basis for template selective recognition has been explored using a quantum chemical approach, providing new insights about the mechanisms underlying template recognition. Thus, a magnetic molecularly imprinted solid-phase extraction method was developed allowing the extraction of dimethoate from spiked olive oil samples, at levels similar to the maximum residue limits imposed by legislation, followed by the quantification of their levels by high-performance liquid chromatography with diode-array detection. Recoveries of 94.55% were obtained, with relative standard deviations lower than 0.53% (n = 3). The developed sample preparation technique enables a selective pre-concentration/enrichment of dimethoate from olive oil matrix with minimum handling and less solvent consumption.
KW - Food
KW - Olive oil
KW - Dimethoate
KW - Sample preparation
KW - Molecularly imprinted polymers
KW - Magnetic responsiveness
KW - Magnetic molecularly imprinted polymers
U2 - 10.1016/j.foodchem.2018.02.003
DO - 10.1016/j.foodchem.2018.02.003
M3 - Article
C2 - 29548458
VL - 254
SP - 309
EP - 316
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
ER -