A liquid chromatography/electrospray ionisation tandem mass spectrometry method for the simultaneous quantification of salicylic, jasmonic and abscisic acids in Coffea arabica leaves

Marta de Sá, João P. Ferreira, Vagner T. Queiroz, Luís Vilas-Boas, Maria C. Silva, Maria H. Almeida, Leonor Guerra-Guimarães, Maria do Rosário Bronze

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

BACKGROUND: Plants have developed an efficient system of recognition that induces a complex network of signalling molecules such as salicylic acid (SA), jasmonic acid (JA) and abscisic acid (ABA) in case of a pathogenic infection. The use of specific and sensitive methods is mandatory for the analysis of compounds in these complex samples. RESULTS: In this study a liquid chromatography/electrospray ionisation tandem mass spectrometry method was developed and validated for the simultaneous quantification of SA, JA and ABA in Coffea arabica (L.) leaves in order to understand the role of these phytohormones in the signalling network involved in the coffee defence response against Hemileia vastatrix. The results showed that the method was specific, linear (r ≥ 0.99) in the range 0.125-1.00 μg mL-1 for JA and ABA and 0.125-5.00 μg mL-1 for SA, and precise (relative standard deviation ≤11%), and the limit of detection (0.010 μg g-1 fresh weight) was adequate for quantifying these phytohormones in this type of matrix. CONCLUSION: In comparison with healthy leaves, those infected with H. vastatrix (resistance reaction) displayed an increase in SA level 24 h after inoculation, suggesting the involvement of an SA-dependent pathway in coffee resistance.

Original languageEnglish
Pages (from-to)529-536
Number of pages8
JournalJournal of the Science of Food and Agriculture
Volume94
Issue number3
DOIs
Publication statusPublished - Feb 2014

Keywords

  • Coffea sp
  • Coffee rust
  • Hemileia vastatrix
  • HPLC/ESI-MS/MS
  • Phytohormones
  • Signalling

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