The complete determination of all rate and equilibrium constants of the network of reversible chemical reactions involving the anthocyanin analogue, 3',4'-dihydroxy-7-O-β-D-glucopyranosyloxyflavylium chloride, was achieved by means of UV-visible spectroscopy, flash photolysis and pH jumps monitored by stopped-flow. An energy level diagram containing all the data was obtained. A detailed step by step procedure illustrating all the calculations is reported.
|Journal||Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology|
|Publication status||Published - 1 Jan 2013|