A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

Jacinto Gomes; Marcos Santos; Ana Amaro; Isabel Pereira da Fonseca; Gabriela Santos-Gomes; João Inácio

doi:10.1016/j.mcp.2016.12.006

A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

Jacinto Gomes, Marcos Santos, Ana Amaro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes, João Inácio

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.

Original language	English
Pages (from-to)	61-64
Number of pages	4
Journal	Molecular and Cellular Probes
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.mcp.2016.12.006
Publication status	Published - 1 Feb 2017

Keywords

Bovine theileriosis
LAMP
Molecular diagnostics
Real-time PCR
Tams1
Theileria annulata

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mcp.2016.12.006

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abstract = "A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.",

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AU - Gomes, Jacinto

AU - Santos, Marcos

AU - Amaro, Ana

AU - Pereira da Fonseca, Isabel

AU - Santos-Gomes, Gabriela

AU - Inácio, João

PY - 2017/2/1

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KW - Real-time PCR

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A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

Abstract

Keywords

UN SDGs

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