A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

Jacinto Gomes, Marcos Santos, Ana Amaro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes, João Inácio

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.

Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalMolecular and Cellular Probes
Volume31
Issue number1
DOIs
Publication statusPublished - 1 Feb 2017

Keywords

  • Bovine theileriosis
  • LAMP
  • Molecular diagnostics
  • Real-time PCR
  • Tams1
  • Theileria annulata

UN Sustainable Development Goals (SDGs)

  • SDG 3 - Good Health and Well-Being

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