TY - JOUR
T1 - A diversity of resistance sources to Fusarium oxysporum f. sp. pisi found within grass pea germplasm
AU - Sampaio, Ana Margarida
AU - Vitale, Stefania
AU - Turrà, David
AU - Di Pietro, Antonio
AU - Rubiales, Diego
AU - van Eeuwijk, Fred
AU - Vaz Patto, Maria Carlota
N1 - Funding Information:
The authors are thankful to Priscila Pereira (ITQB NOVA) for grass pea seed multiplication, Carmen Santos (ITQB NOVA) for collaboration in fungal inoculation, Esther Aguilera and T?nia Fernandes (Universidad de C?rdoba) for the help with the Fop -GFP protoplast transformation and Mariana Ferreira (ITQB NOVA) for the confocal laser scanning microscopy images acquisition.
Funding Information:
Financial support by Fundação para a Ciência e Tecnologia (FCT), Portugal, is acknowledge through grant PD/BD/114418/2016 (AMS), the research unit GREEN-IT (UID/04551/2020), the QuaLaty project (PTDC/AGR-TEC/0992/2014), and the Spanish Research Agency (AEI) projects AGL2017-82907-R and BIO2016-78923-R. We also thank to the FP7 EU funding through the LEGATO project (grant agreement n°FP7-613551). This work was partially supported by PPBI- Portuguese Platform of BioImaging (PPBI-POCI-01-0145-FEDER-022122) co-funded by national funds from OE – “Orçamento de Estado” and by European funds from FEDER - “Fundo Europeu de Desenvolvimento Regional”.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG part of Springer Nature.
PY - 2021/6
Y1 - 2021/6
N2 - Purpose: Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fop and characterize the detected resistance mechanisms. Methods: A grass pea worldwide collection of accessions was characterized under controlled conditions for response to Fop race 2. Fungal colonization dynamics and potential resistance mechanisms were studied through confocal laser scanning microscopy (CLSM) using Fop race 2 expressing green fluorescent protein (GFP). Results: A quantitative nature of resistance to Fop, ranging from highly to partially resistant and susceptible accessions was detected, with resistance being the most frequent phenotype. Diverse colonization patterns were observed, suggesting the existence of different resistance mechanisms. In the highly resistant accessions, absence of fungal colonization in the vascular tissue was detected, while fungal progression was arrested at the level of roots both in highly resistant and partially resistant accessions. Conclusions: The resistant accessions identified here can be exploited in grass pea breeding for fusarium wilt resistance, and due to the phylogenetic relatedness to pea, potentially contribute to pea improvement.
AB - Purpose: Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fop and characterize the detected resistance mechanisms. Methods: A grass pea worldwide collection of accessions was characterized under controlled conditions for response to Fop race 2. Fungal colonization dynamics and potential resistance mechanisms were studied through confocal laser scanning microscopy (CLSM) using Fop race 2 expressing green fluorescent protein (GFP). Results: A quantitative nature of resistance to Fop, ranging from highly to partially resistant and susceptible accessions was detected, with resistance being the most frequent phenotype. Diverse colonization patterns were observed, suggesting the existence of different resistance mechanisms. In the highly resistant accessions, absence of fungal colonization in the vascular tissue was detected, while fungal progression was arrested at the level of roots both in highly resistant and partially resistant accessions. Conclusions: The resistant accessions identified here can be exploited in grass pea breeding for fusarium wilt resistance, and due to the phylogenetic relatedness to pea, potentially contribute to pea improvement.
KW - Fusarium oxysporum
KW - Grass pea
KW - Quantitative resistance
KW - Resistance mechanisms
UR - http://www.scopus.com/inward/record.url?scp=85101850312&partnerID=8YFLogxK
U2 - 10.1007/s11104-021-04895-z
DO - 10.1007/s11104-021-04895-z
M3 - Article
AN - SCOPUS:85101850312
SN - 0032-079X
VL - 463
SP - 19
EP - 38
JO - Plant and Soil
JF - Plant and Soil
IS - 1-2
ER -