A continuous system for biocatalytic hydrogenation of CO2 to formate

Sofia M. da Silva, Ines Pereira, Cláudia Mourato, Mónica Martins

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this work a novel bioprocess for hydrogenation of CO2 to formate was developed, using whole cell catalysis by a sulfate-reducing bacterium. Three Desulfovibrio species were tested (D. vulgaris Hildenborough, D. alaskensis G20, and D. desulfuricans ATCC 27774), of which D. desulfuricans showed the highest activity, producing 12�mM of formate in batch, with a production rate of 0.09�mM�h−1. Gene expression analysis indicated that among the three formate dehydrogenases and five hydrogenases, the cytoplasmic FdhAB and the periplasmic [FeFe] HydAB are the main enzymes expressed in D. desulfuricans in these conditions. The new bioprocess for continuous formate production by D. desulfuricans had a maximum specific formate production rate of 14�mM�gdcw −1�h−1, and more than 45�mM of formate were obtained with a production rate of 0.40�mM�h−1. This is the first report of a continuous process for biocatalytic formate production.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalBioresource Technology
Volume235
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Formate production
  • H storage
  • Hydrogenation of CO
  • Sulfate reducing bacteria
  • Whole cell biocatalysis

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