A computational and experimental study to develop E-selectin targeted peptides for molecular imaging applications

Ana Carina Fernandes, Mengjiao Liu, Teresa Sorbo, Lia Christina Appold, Marianne Ilbert, Géraldine Ferracci, Fabian Kiessling, Ricardo J. F. Branco, Twan Lammers, Olga Iranzo

Research output: Contribution to journalArticle

Abstract

Aim: E-selectin is overexpressed on angiogenic and inflamed endothelium. Molecules binding to E-selectin with high affinity and specificity enable its use as a molecular imaging biomarker. Material & methods: The interactions of four different peptides (i.e., Ac-P1 [Acetyl-IELLQAR-CONH 2 ], H 2 N-P2 [H 2 N-DITWDQLWDLMK-CONH 2 ], H 2 N-P3A5 [H 2 N-YRNWAGRW-CONH 2 ], and Ac-P4 [Acetyl-YRNWDGRW-CONH 2 ]) with E-selectin were analyzed by computational methodologies, surface plasmon resonance and in vitro using activated human umbilical vein endothelial cells. Poly(butyl cyanoacrylate) microbubbles were functionalized with the best candidates and evaluated as molecular ultrasound probes in cultured cells and explanted carotid arteries. Results: H 2 N-P3A5 and Ac-P4 peptides bound stronger to E-selectin than Ac-P1 and H 2 N-P2, but with lower specificity. H 2 N-P2 bound with higher specificity and affinity than Ac-P1. Conclusion: H 2 N-P2 is a good candidate for designing E-selectin-targeted molecular imaging agents.

Original languageEnglish
Pages (from-to)2695-2711
Number of pages17
JournalFuture Medicinal Chemistry
Volume10
Issue number23
DOIs
Publication statusPublished - 1 Dec 2018

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Keywords

  • Angiogenesis
  • E-selectin
  • Microbubble
  • Molecular imaging
  • Molecular modelling
  • Peptides
  • Ultrasound

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