@article{cde6d47759e343d4b7fd470a10e98cd4,
title = "A computational and experimental study to develop E-selectin targeted peptides for molecular imaging applications",
abstract = " Aim: E-selectin is overexpressed on angiogenic and inflamed endothelium. Molecules binding to E-selectin with high affinity and specificity enable its use as a molecular imaging biomarker. Material & methods: The interactions of four different peptides (i.e., Ac-P1 [Acetyl-IELLQAR-CONH 2 ], H 2 N-P2 [H 2 N-DITWDQLWDLMK-CONH 2 ], H 2 N-P3A5 [H 2 N-YRNWAGRW-CONH 2 ], and Ac-P4 [Acetyl-YRNWDGRW-CONH 2 ]) with E-selectin were analyzed by computational methodologies, surface plasmon resonance and in vitro using activated human umbilical vein endothelial cells. Poly(butyl cyanoacrylate) microbubbles were functionalized with the best candidates and evaluated as molecular ultrasound probes in cultured cells and explanted carotid arteries. Results: H 2 N-P3A5 and Ac-P4 peptides bound stronger to E-selectin than Ac-P1 and H 2 N-P2, but with lower specificity. H 2 N-P2 bound with higher specificity and affinity than Ac-P1. Conclusion: H 2 N-P2 is a good candidate for designing E-selectin-targeted molecular imaging agents. ",
keywords = "Angiogenesis, E-selectin, Microbubble, Molecular imaging, Molecular modelling, Peptides, Ultrasound",
author = "Fernandes, {Ana Carina} and Mengjiao Liu and Teresa Sorbo and Appold, {Lia Christina} and Marianne Ilbert and G{\'e}raldine Ferracci and Fabian Kiessling and Branco, {Ricardo J. F.} and Twan Lammers and Olga Iranzo",
note = "info:eu-repo/grantAgreement/FCT/3599-PPCDT/128542/PT# info:eu-repo/grantAgreement/FCT/5876/147258/PT# info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F69163%2F2010/PT# The authors gratefully acknowledge financial support by the Fundacao para a Ciencia e a Tecnologia (FCT; Project PTDC/QEQ-MED/2656/2012), the national funds FCT/MEC (UID/Multi/04378/2013), the DAAD-Campus France (Project PHC Procope 31017PH), the European Research Council (ERC Starting Grant 309495: NeoNaNo), the European Commission (H2020 Euro-NanoMed III: NSC4DIPG), and the German Research Foundation (DFG: KI1072/11-1, LA2937/1-2 and GRK 2375). RJFB acknowledges the FCT (Fellowship SFRH/BPD/69163/2010), TS acknowledges the Federico II University of Naples (Erasmus grant) and ML acknowledges China Scholarship Council (CSC: Grant number 201506910070). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.",
year = "2018",
month = dec,
day = "1",
doi = "10.4155/fmc-2018-0244",
language = "English",
volume = "10",
pages = "2695--2711",
journal = "Future Medicinal Chemistry",
issn = "1756-8919",
publisher = "Future Science",
number = "23",
}