A BolA-like morphogene from the alga Chlamydomonas reinhardtii changes morphology and induces biofilm formation in Escherichia coli

Cecilia Maria Arraiano

doi:10.1111/1574-6968.12051

A BolA-like morphogene from the alga Chlamydomonas reinhardtii changes morphology and induces biofilm formation in Escherichia coli

Cecilia Maria Arraiano

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article

9 Citations (Scopus)

Original language	Unknown
Pages (from-to)	39-47
Journal	FEMS Microbiology Letters
Volume	339
Issue number	1
DOIs	https://doi.org/10.1111/1574-6968.12051
Publication status	Published - 1 Jan 2013

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10.1111/1574-6968.12051

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@article{27ac7f7285ef4609827f97699b16b054,

title = "A BolA-like morphogene from the alga Chlamydomonas reinhardtii changes morphology and induces biofilm formation in Escherichia coli",

abstract = "Escherichia coli BolA protein is a stress-inducible morphogene, regulates transcription, forms biofilms and interacts with monothiol glutaredoxins. Its presence has been documented in plants but its role remains enigmatic. This study attempts to functionally dissect the role of a BolA-domain-containing protein in the alga Chlamydomonas reinhardtii. Of the five C. reinhardtii bolA-like genes annotated for the presence of BolA-domain, the open reading frame with the highest similarity to algal systems was cloned and the protein over-expressed in E. coli. This over-expression did not affect E. coli growth but induced biofilm formation and changed its morphology, indicating functional conservancy. This is the first compelling evidence depicting the role of a plant BolA-like protein in morphogenetic pathway and biofilm formation. The implications of the phenotypic consequences of this heterologous expression are discussed.",

keywords = "BolA, morphogene, biofilm, Chlamydomonas reinhardtii, palmelloid, round morphology",

author = "Arraiano, {Cecilia Maria}",

year = "2013",

month = jan,

day = "1",

doi = "10.1111/1574-6968.12051",

language = "Unknown",

volume = "339",

pages = "39--47",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Blackwell Publishing Ltd",

number = "1",

}

TY - JOUR

T1 - A BolA-like morphogene from the alga Chlamydomonas reinhardtii changes morphology and induces biofilm formation in Escherichia coli

AU - Arraiano, Cecilia Maria

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Escherichia coli BolA protein is a stress-inducible morphogene, regulates transcription, forms biofilms and interacts with monothiol glutaredoxins. Its presence has been documented in plants but its role remains enigmatic. This study attempts to functionally dissect the role of a BolA-domain-containing protein in the alga Chlamydomonas reinhardtii. Of the five C. reinhardtii bolA-like genes annotated for the presence of BolA-domain, the open reading frame with the highest similarity to algal systems was cloned and the protein over-expressed in E. coli. This over-expression did not affect E. coli growth but induced biofilm formation and changed its morphology, indicating functional conservancy. This is the first compelling evidence depicting the role of a plant BolA-like protein in morphogenetic pathway and biofilm formation. The implications of the phenotypic consequences of this heterologous expression are discussed.

AB - Escherichia coli BolA protein is a stress-inducible morphogene, regulates transcription, forms biofilms and interacts with monothiol glutaredoxins. Its presence has been documented in plants but its role remains enigmatic. This study attempts to functionally dissect the role of a BolA-domain-containing protein in the alga Chlamydomonas reinhardtii. Of the five C. reinhardtii bolA-like genes annotated for the presence of BolA-domain, the open reading frame with the highest similarity to algal systems was cloned and the protein over-expressed in E. coli. This over-expression did not affect E. coli growth but induced biofilm formation and changed its morphology, indicating functional conservancy. This is the first compelling evidence depicting the role of a plant BolA-like protein in morphogenetic pathway and biofilm formation. The implications of the phenotypic consequences of this heterologous expression are discussed.

KW - BolA

KW - morphogene

KW - biofilm

KW - Chlamydomonas reinhardtii

KW - palmelloid

KW - round morphology

U2 - 10.1111/1574-6968.12051

DO - 10.1111/1574-6968.12051

M3 - Article

VL - 339

SP - 39

EP - 47

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -