TY - JOUR
T1 - 4-MUF-NAG for fungal biomass determination
T2 - Scope and limitations in the context of biodeterioration studies
AU - Sequeira, Sílvia Oliveira
AU - Coutinho, Mathilda L.
AU - Lima, João C.
AU - Phillips, Alan J L
AU - Macedo, Maria F.
AU - Cabrita, Eurico J.
N1 - Sem PDF conforme despacho.
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F72216%2F2010/PT#
info:eu-repo/grantAgreement/FCT/5876/147238/PT#
info:eu-repo/grantAgreement/FCT/5876/147258/PT#
Funding: this study was supported by Fundacao para a Ciencia e Tecnologia - Ministerio da Educacao e Ciencia (FCT-MEC), Portugal, with a doctoral grant [SFRH/BD/72216/2010] and the project UID/EAT/00729/2013; and by Unidade de Ciencias Biomoleculares Aplicadas - UCIBIO which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).
PY - 2016/11/1
Y1 - 2016/11/1
N2 - In the field of biodeterioration of cultural heritage by fungi, quantification of fungal growth is a regularly required procedure. Recently, a fluorometric method for detection and estimation of fungal growth using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) was proposed. In order to evaluate the potentials and limitations of this method for fungal biomass quantification, different experimental conditions to develop calibration curves were tested, using Aspergillus niger, Cladosporium cladosporioides, Chaetomium globosum and Penicillium chrysogenum fungal strains. The results showed that the correlation between fluorescence and biomass differs according to fungal species and stage of growth. Therefore, a measurement of fluorescence may not be indicative of a specific value of biomass. Also, false positive and negative results for fungal growth can be obtained. C. globosum was the fungal species that showed the most constant correlation between biomass and fluorescence throughout the different incubation periods.
AB - In the field of biodeterioration of cultural heritage by fungi, quantification of fungal growth is a regularly required procedure. Recently, a fluorometric method for detection and estimation of fungal growth using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) was proposed. In order to evaluate the potentials and limitations of this method for fungal biomass quantification, different experimental conditions to develop calibration curves were tested, using Aspergillus niger, Cladosporium cladosporioides, Chaetomium globosum and Penicillium chrysogenum fungal strains. The results showed that the correlation between fluorescence and biomass differs according to fungal species and stage of growth. Therefore, a measurement of fluorescence may not be indicative of a specific value of biomass. Also, false positive and negative results for fungal growth can be obtained. C. globosum was the fungal species that showed the most constant correlation between biomass and fluorescence throughout the different incubation periods.
KW - 4-MUF-NAG
KW - Fungal growth
KW - NAGase
KW - Spectrofluorometry
UR - http://www.scopus.com/inward/record.url?scp=84996671061&partnerID=8YFLogxK
U2 - 10.1016/j.culher.2016.06.002
DO - 10.1016/j.culher.2016.06.002
M3 - Article
AN - SCOPUS:84996671061
SN - 1296-2074
VL - 22
SP - 992
EP - 998
JO - Journal of Cultural Heritage
JF - Journal of Cultural Heritage
ER -