Abstract

In the field of biodeterioration of cultural heritage by fungi, quantification of fungal growth is a regularly required procedure. Recently, a fluorometric method for detection and estimation of fungal growth using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) was proposed. In order to evaluate the potentials and limitations of this method for fungal biomass quantification, different experimental conditions to develop calibration curves were tested, using Aspergillus niger, Cladosporium cladosporioides, Chaetomium globosum and Penicillium chrysogenum fungal strains. The results showed that the correlation between fluorescence and biomass differs according to fungal species and stage of growth. Therefore, a measurement of fluorescence may not be indicative of a specific value of biomass. Also, false positive and negative results for fungal growth can be obtained. C. globosum was the fungal species that showed the most constant correlation between biomass and fluorescence throughout the different incubation periods.

Original languageEnglish
Pages (from-to)992-998
Number of pages7
JournalJournal of Cultural Heritage
Volume22
DOIs
Publication statusPublished - 1 Nov 2016

Fingerprint

Biomass
Fluorescence
quantification
Niger
Aspergillus
Fungi
cultural heritage
Calibration
Biodeterioration
Values
Quantification

Keywords

  • 4-MUF-NAG
  • Fungal growth
  • NAGase
  • Spectrofluorometry

Cite this

@article{e32af1a10927455398518cd3c0df97f5,
title = "4-MUF-NAG for fungal biomass determination: Scope and limitations in the context of biodeterioration studies",
abstract = "In the field of biodeterioration of cultural heritage by fungi, quantification of fungal growth is a regularly required procedure. Recently, a fluorometric method for detection and estimation of fungal growth using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) was proposed. In order to evaluate the potentials and limitations of this method for fungal biomass quantification, different experimental conditions to develop calibration curves were tested, using Aspergillus niger, Cladosporium cladosporioides, Chaetomium globosum and Penicillium chrysogenum fungal strains. The results showed that the correlation between fluorescence and biomass differs according to fungal species and stage of growth. Therefore, a measurement of fluorescence may not be indicative of a specific value of biomass. Also, false positive and negative results for fungal growth can be obtained. C. globosum was the fungal species that showed the most constant correlation between biomass and fluorescence throughout the different incubation periods.",
keywords = "4-MUF-NAG, Fungal growth, NAGase, Spectrofluorometry",
author = "Sequeira, {S{\'i}lvia Oliveira} and Coutinho, {Mathilda L.} and Lima, {Jo{\~a}o C.} and Phillips, {Alan J L} and Macedo, {Maria F.} and Cabrita, {Eurico J.}",
note = "Sem PDF conforme despacho. info:eu-repo/grantAgreement/FCT/SFRH/SFRH{\%}2FBD{\%}2F72216{\%}2F2010/PT# info:eu-repo/grantAgreement/FCT/5876/147238/PT# info:eu-repo/grantAgreement/FCT/5876/147258/PT# Funding: this study was supported by Fundacao para a Ciencia e Tecnologia - Ministerio da Educacao e Ciencia (FCT-MEC), Portugal, with a doctoral grant [SFRH/BD/72216/2010] and the project UID/EAT/00729/2013; and by Unidade de Ciencias Biomoleculares Aplicadas - UCIBIO which is financed by national funds from FCT/MEC (UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728).",
year = "2016",
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T1 - 4-MUF-NAG for fungal biomass determination

T2 - Scope and limitations in the context of biodeterioration studies

AU - Sequeira, Sílvia Oliveira

AU - Coutinho, Mathilda L.

AU - Lima, João C.

AU - Phillips, Alan J L

AU - Macedo, Maria F.

AU - Cabrita, Eurico J.

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PY - 2016/11/1

Y1 - 2016/11/1

N2 - In the field of biodeterioration of cultural heritage by fungi, quantification of fungal growth is a regularly required procedure. Recently, a fluorometric method for detection and estimation of fungal growth using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) was proposed. In order to evaluate the potentials and limitations of this method for fungal biomass quantification, different experimental conditions to develop calibration curves were tested, using Aspergillus niger, Cladosporium cladosporioides, Chaetomium globosum and Penicillium chrysogenum fungal strains. The results showed that the correlation between fluorescence and biomass differs according to fungal species and stage of growth. Therefore, a measurement of fluorescence may not be indicative of a specific value of biomass. Also, false positive and negative results for fungal growth can be obtained. C. globosum was the fungal species that showed the most constant correlation between biomass and fluorescence throughout the different incubation periods.

AB - In the field of biodeterioration of cultural heritage by fungi, quantification of fungal growth is a regularly required procedure. Recently, a fluorometric method for detection and estimation of fungal growth using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) was proposed. In order to evaluate the potentials and limitations of this method for fungal biomass quantification, different experimental conditions to develop calibration curves were tested, using Aspergillus niger, Cladosporium cladosporioides, Chaetomium globosum and Penicillium chrysogenum fungal strains. The results showed that the correlation between fluorescence and biomass differs according to fungal species and stage of growth. Therefore, a measurement of fluorescence may not be indicative of a specific value of biomass. Also, false positive and negative results for fungal growth can be obtained. C. globosum was the fungal species that showed the most constant correlation between biomass and fluorescence throughout the different incubation periods.

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KW - NAGase

KW - Spectrofluorometry

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