TY - JOUR
T1 - 3D-printed biosurfactant-chitosan antibacterial coating for the prevention of silicone-based associated infections
AU - Narciso, Francisco
AU - Cardoso, Sara
AU - Monge, Nuno
AU - Lourenço, Madalena
AU - Martin, Victor
AU - Duarte, Noélia
AU - Santos, Catarina
AU - Gomes, Pedro
AU - Bettencourt, Ana
AU - Ribeiro, Isabel A. C.
N1 - Funding Information:
The authors would like to thank the Portuguese government, Fundação para a Ciência e Tecnologia (FCT), for the financial support through national funds PTDC/BTM-SAL/29335/2017, under iMed.ULisboa UIDB/04138/2020 and UIDP/04138/2020 and under IPL IPL/2022/3DWounDres_ESELx. FCT is also acknowledged for the research initiation Grant BII/2/FF/2022. The authors acknowledge the financial support from FCT and Portugal 2020 to the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa – RNEM; LISBOA-01-0145-FEDER-402-022125). The authors also acknowledge Andreia Bento da Silva (FFUL) for the assistance with the HPLC-MS equipment and Isabel Nogueira (IST Microlab) for the assistance with SEM imaging.
Funding Information:
The authors would like to thank the Portuguese government, Fundação para a Ciência e Tecnologia ( FCT ), for the financial support through national funds PTDC/BTM-SAL/29335/2017 , under iMed.ULisboa UIDB/04138/2020 and UIDP/04138/2020 and under IPL IPL/2022/3DWounDres_ESELx. FCT is also acknowledged for the research initiation Grant BII/2/FF/2022 . The authors acknowledge the financial support from FCT and Portugal 2020 to the Portuguese Mass Spectrometry Network (Rede Nacional de Espectrometria de Massa – RNEM; LISBOA-01-0145-FEDER-402-022125 ). The authors also acknowledge Andreia Bento da Silva (FFUL) for the assistance with the HPLC-MS equipment and Isabel Nogueira (IST Microlab) for the assistance with SEM imaging.
Publisher Copyright:
© 2023 The Authors
PY - 2023/10
Y1 - 2023/10
N2 - Infections associated with the surfaces of medical devices represent a critical problem due to biofilm formation and the growing resistance towards antibacterial drugs. This is particularly relevant in commonly used invasive devices such as silicone-based ones where a demand for alternative antibiofilm surfaces is increasing. In this work, an antimicrobial chitosan-biosurfactant hydrogel mesh was produced by 3D-printing. The 3D structure was designed to coat polydimethylsiloxane-based medical devices for infection prevention. Additionally, the porous 3D structure allows the incorporation of customized bioactive components. For this purpose, two biosurfactants (surfactin and sophorolipids) were biosynthesized and tested for their antimicrobial activity. In addition, the printing of surfactant-chitosan-based coatings was optimized, and the resulting 3D structures were characterized (i.e., wettability, FTIR-ATR, antimicrobial activity, and biocompatibility). Compared with surfactin, the results showed a better yield and higher antibacterial activity against Gram-positive bacteria for sophorolipids (SLs). Thus, SLs were used to produce chitosan-based 3D-printed coatings. Overall, the SLs-impregnated coatings showed the best antibacterial activity against Staphylococcus aureus planktonic bacteria (61 % of growth inhibition) and antibiofilm activity (2 log units reduction) when compared to control. Furthermore, concerning biocompatibility, the coatings were cytocompatible towards human dermal fibroblasts. Finally, the coating presented a mesh suitable to be filled with a model bioactive compound (i.e., hyaluronic acid), paving the way to be used for customized therapeutics.
AB - Infections associated with the surfaces of medical devices represent a critical problem due to biofilm formation and the growing resistance towards antibacterial drugs. This is particularly relevant in commonly used invasive devices such as silicone-based ones where a demand for alternative antibiofilm surfaces is increasing. In this work, an antimicrobial chitosan-biosurfactant hydrogel mesh was produced by 3D-printing. The 3D structure was designed to coat polydimethylsiloxane-based medical devices for infection prevention. Additionally, the porous 3D structure allows the incorporation of customized bioactive components. For this purpose, two biosurfactants (surfactin and sophorolipids) were biosynthesized and tested for their antimicrobial activity. In addition, the printing of surfactant-chitosan-based coatings was optimized, and the resulting 3D structures were characterized (i.e., wettability, FTIR-ATR, antimicrobial activity, and biocompatibility). Compared with surfactin, the results showed a better yield and higher antibacterial activity against Gram-positive bacteria for sophorolipids (SLs). Thus, SLs were used to produce chitosan-based 3D-printed coatings. Overall, the SLs-impregnated coatings showed the best antibacterial activity against Staphylococcus aureus planktonic bacteria (61 % of growth inhibition) and antibiofilm activity (2 log units reduction) when compared to control. Furthermore, concerning biocompatibility, the coatings were cytocompatible towards human dermal fibroblasts. Finally, the coating presented a mesh suitable to be filled with a model bioactive compound (i.e., hyaluronic acid), paving the way to be used for customized therapeutics.
KW - Additive manufacturing
KW - Mesh
KW - PDMS
KW - Polymer-coating
KW - Sophorolipids
KW - Surfactin
UR - http://www.scopus.com/inward/record.url?scp=85167595705&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2023.113486
DO - 10.1016/j.colsurfb.2023.113486
M3 - Article
C2 - 37572399
AN - SCOPUS:85167595705
SN - 0927-7765
VL - 230
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
M1 - 113486
ER -
