MEMBRANE TRAFFIC IN CARDIOVASCULAR PATHOLOGIES AND  INFECTION

Curriculum Vitae : Otilia Vieira

1. EDUCATION

2. CURRENT AND PREVIOUS SCIENTIFIC AND/OR PROFESSIONAL POSITIONS

3. FELLOWSHIPS AND AWARDS:

2012: Arrisca C2011- ACIC AWARD for the best business ideas, Univ. of Coimbra.

2011: “Bolsa de Ignição” for Scientific projects with high potential to start a company, Univ. Coimbra.

2004-2005: Max-Planck Society post-doctoral fellowship.

2002-2004: Marie-Curie Post-doctoral Fellow of the European Community.

2002: Post-Doctoral EMBO Fellowship. Declined after acceptance of the Marie-Curie Fellowship.

2000-2002: Post-doctoral Fellow from Foundation for Science and Technology (FCT), Portugal

1995-1999: PhD fellowship from FCT.

1997: Merck Sharpe & Dohme Prize for the presentation entitled "Antioxidation of the Human Low density lipoproteins Antioxidação. New Biochemical mechanisms for dietary compounds", at the VII Congresso Português de Aterosclerose, Luso (Portugal).

1993-1995: MSc fellowship from FCT

1993: Awarded the "Sociedade Farmacêutica Lusitana" Prize for the highest classified student finishing the Licenciate course in Pharmacy at the University of Coimbra.

4. SCIENTIFIC INTERESTS

Surfactants in the prophylaxis against sexually transmitted and vertically transmitted urogenital infections, phagosomal maturation, etc.

5. FUNDING

Total grant portfolio > € 1.000.000,00 including an international consortium with Harvard Medical School, USA.

6. SUPERVISION OF GRADUATE STUDENTS ANS POSTDOCS

5 Postdocs. C. Cardoso, now researcher at Crioestaminal, Biocant Portugal. L. Jordão, now researcher at INSA, Lisbon Portugal. L. Estronca, now postdoc at Biocant. D. Mateus and M. Rego (still ongoing).

4 PhD students. A. Inácio, now postdoc at CNC. M. Viegas, now postdoc at Rio Grande do Sul (Brazil). I. Santarino and N. Domingues (still ongoing).

Co-supervisor of 4 PhD students

7. ORGANIZATION OF NATIONAL AND INTERNATIONAL MEETINGS

2013: Organizer of the Advanced Course in “Membrane Traffic and Disease”, Univ. of Coimbra, http://beb.cnbc.pt/det_courses.asp?id=588

2010: Organizer of the Advanced Course in “Cell Biology of Infection”, Univ. of Coimbra, http://beb.cnbc.pt/det_courses.asp?id=450

2008: Organizer of the Advanced Course in “Cell Biology of Infection”, Univ. of Coimbra, http://beb.cnbc.pt/det_courses.asp?id=244

1998: Co-organizer, of the Advanced Course in “Cell Toxicity Triggered by Human Oxidized Low Density Lipoproteins (LDL)”, Univ. of Coimbra

8. PUBLICATIONS

33 peer-review publications (12 as first author; 11 as last author and 12 as corresponding author) with circa of 3174 citations and an h-factor of 23.

1.      J.A.N. Laranjinha, O. Vieira, V.M.C. Madeira, and L.M. Almeida (1995). Two related phenolic antioxidants with opposite effects on vitamin E content in low density lipoproteins. Consumption versus regeneration. Arch. Biochem. Biophys. 323:373-381.

2.      J.A.N. Laranjinha, O. Vieira, V.M.C. Madeira, and L.M. Almeida (1996). Inhibition of metmyoglobin/H2O2-dependent LDL lipid peroxidation by naturally ocurring phenolic acids. Biochem. Pharmacol. 51:395-402.

3.      O. Vieira, J.A.N. Laranjinha, V.M.C. Madeira, and L.M. Almeida (1996). A rapid isolation of low density lipoproteins in a concentrated fraction free from water-soluble plasma antioxidants. J. Lipid Res. 37:379-385.

4.      O. Vieira, I. Blanc, J.A.N. Laranjinha, L.M. Almeida, R. Salvayre, and A. Nègre-Salvayre (1998). Effect of Dietary phenolic compounds on apoptosis of human cultured endothelial cells induced by oxidized LDL. Br. J. Pharmacol.123:565-573.

5.      O. Vieira, J.A.N. Laranjinha, V.M.C. Madeira, and L.M. Almeida (1998). Cholesteryl ester hydroperoxide formation in myoglobin-catalyzed low density lipoprotein oxidation - Concerted antioxidant activity of caffeic and p-coumaric acids with ascorbate. Biochem. Pharmacol. 55:333-340.

6.      O. Vieira, I. Escargueil-Blanc, G. Jürgens, C. Borner, L. Almeida, R. Salvayre, and A. Nègre-Salvayre (2000). Oxidized LDL alter the activity of the ubiquitin-proteasome pathway. Potential role in LDL-induced apoptosis. FASEB J.14(3):532-542.

7.      N. Vacaresse*, O. Vieira*, F. Robbesyn, G. Jurgens, R. Salvayre, and A. Negre-Salvayre (2001). Phenolic antioxidants trolox and caffeic acid modulate the oxidized LDL-induced EGF-receptor activation. (* equally contributed). Br. J. Pharmacol.132(8):1777-1788.

8.      O.V. Vieira, R.J. Botelho, L. Rameh, S.M. Brachmann, T. Matsuo, H.W. Davidson, A. Schreiber, J.M. Backer, L. Cantley, and S. Grinstein (2001).^* Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation.J. Cell Biol. 155(1):19-25.

* Comment in J. Cell Biol. 155 (1):15-17. Exceptional rating in Faculty of 1000 Biology.

9.      M.R. Terebiznik, O.V.Vieira, S. Marcus, W.S. Trimble, T. Meyer, B.B. Finlay, and S. Grinstein (2002)*. Elimination of host cell PI(4,5)P2 by the phosphatase SopB/SigD promotes membrane fission during invasion by Salmonella. Nature Cell Biol.4(10):766-773.

* Comment in J. Cell Biol. 159(2):200. Exceptional rating in Faculty of 1000 Biology.

10.    O.V. Vieira, C. Bucci, R. Harrison, W.S. Trimble, L. Lanzetti, J. Gruenberg, A. D. Schreiber, P.D. Stahl, and S. Grinstein (2003). Modulation of Rab5 and Rab7 recruitment to phagosomes by phosphatidyl-inositol 3-kinase. Mol. Cell. Biol. 23(7):2501-2514.

11.    F. Robbesyn, V. Garcia, N. Auge, O. Vieira, M.F. Frisach, R. Salvayre, and A. Negre-Salvayre (2003). HDL couterbalance the proinflammatory effect of oxidized LDL by inhibiting intracellular reactive oxygen species rise, proteasome activation and subsequent NF-kappaB activation in smooth muscle cells. FASEB J.17(6):743-5.

12.    R. Harrisson, C. Bucci, O.V. Vieira, T.A. Schroer, and S. Grinstein (2003). Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules:role of Rab7 and RILP. Mol. Cell. Biol.23(18):6494-6506.

13.    O.V. Vieira, R. Harrison, C. Scott, H. Stenmark, D. Alexander , J. Liu , J. Gruenberg, A.D. Schreiber, and S. Grinstein (2004). Acquisition of Hrs, an essential component of phagosomal maturation, is impaired by mycobacteria. Mol. Cell. Biol. 24(10):4593-4604.

14.    O.V. Vieira, P. Verkade, A. Manninen and K. Simons (2005). FAPP2 is part of the apical transport machinery in polarized MDCK cells. J. Cell Biol.170(4):521-526.

Exceptional rating in Faculty of 1000 Biology.

15.    O.V. Vieira, K. Gaus, P. Verkade, W.L.C. Vaz and K. Simons (2006) * FAPP2, cilium formation and the compartmentalization of the apical membrane in polarized MDCK cells.Proc. Natl. Acad. Sci. USA103(49):18556-18561.

*Comment in Proc. Natl. Acad Sci USA 103(49):18383-18384. This paper was also highlighted in the cover of the Journal.

16.    D. Halter, S. Neumann, S.M. van Dijk, J. Wolthoorn, A.M. de Mazière, O.V. Vieira, P. Mattjus, J. Klumperman, G. van Meer, and H. Sprong (2007). *Pre- and Post-Golgi Translocation of Glucosylceramide in Glycosphingolipid Synthesis.J. Cell Biol.179(1):101-115.

*Spotlight in J. Cell Biol. 179(1):11-13.

17.    O.V. Vieira, D.O. Hartmann, C.M. Cardoso, D. Oberdoerfer, M. Baptista, M. Santos, L. Almeida, J. Ramalho-Santos and W.L.C. Vaz (2008). Surfactants as microbicides and contraceptive agents: a systematic in vitro study. PloS ONE 3(8):e2913.

18.    C.M. Cardoso, L. Jordão and O.V. Vieira¹ (2010). Rab10 is required for phagosome maturation and its overexpression can change the fate of Mycobacterium-containing phagosomes. Traffic 11(2):221-35.

19.    A. Inácio, K. Mesquita, M. Baptista, J. Ramalho-Santos, W.L.C. Vaz and O.V. Vieira¹ (2011). In vitro surfactant structure-toxicity relationships: implications for surfactant use in sexually transmitted infection prophylaxis and contraception. PloS ONE 6(5):e19850.

20.    R. Ferreira, T. Santos, M. Viegas, L. Cortes, A.P. Silva, S. Xapelli, L. Bernardino, O.V. Vieira, and J.O. Malva (2011). Neuropeptide Y inhibits interleukin-1 beta (IL-1β)-induced phagocytosis by microglial cells. J. Neuroinflammation8(169):1-15.

21.    L.M.B.B. Estronca, J. Silva, J. Sampaio, A. Shevchenko, P. Verkade, W.L.C. Vaz and O.V. Vieira (2012). Molecular Etiology of Atherogenesis - In Vitro Induction of Lipidosis in Macrophages with a New LDL Model. PLoS One. 2012;7(4):e34822.

22.    M.S. Viegas, L.M.B.B. Estronca, O.V. Vieira(2012). Comparison of the Kinetics of Maturation of Phagosomes Containing Apoptotic Cells and IgG-Opsonized Particles. PLoS One. 2012;7(10): e48391

23.    A. Inácio, G. Costa, M. S. Santos, A. J. M. Moreno, W.L.C. Vaz and O.V. Vieira (2013). Mitochondrial Dysfunction is the Focus of Quaternary Ammonium Surfactant Toxicity to Mammalian Epithelial Cells. Antimicrobial Agents and Chemotherapy. 2013; 57 (6):2631-9

This article was featured online on Global Medical Discovery [ISSN 1929-8536] (http://globalmedicaldiscovery.com ).

24.    AS Mello, MP Goldim, J Mezzalira J, CS Garcia, VV Daitz, CD Castilhos, MS Viegas, OV Vieira, JC Coelho (2014). LAMP2 as a marker of EBV-mediated B lymphocyte transformation in the study of lysosomal storage diseases. Mol Cell Biochem. 2014; 385:1-6.

25.    A. Silva, L. Naia, A. Dominguez, M. Ribeiro, J. Rodrigues, O.V. Vieira, V. Lessmann and A. C. Rego (2015). Overexpression of BDNF and FL-TrkB receptor ameliorate striatal neural survival in Huntington’s disease. Neurodegener Dis. 2015; 15(4):207-18. doi: 10.1159/000375447

26.    Â. Inácio, N. S. Domingues, A. Nunes, P. T. Martins, M. J. Moreno, L. M. Estronca, R. Fernandes, A. J. M. Moreno, M. J. Borrego, J. P. Gomes, W. L. C. Vaz and O. V. Vieira (2016). Quaternary ammonium surfactant structure determines selective toxicity towards bacteria: mechanisms of action and clinical implications in antibacterial prophylaxis. J Antimicrob Chemother 2016; 71(3):641-54. doi: 10.1093/jac/dkv405

27.    Â. Inácio, A. Nunes, C. Milho C, L. J. Mota, M. J. Borrego, J. P. Gomes, W. L. C. Vaz and O. V. Vieira (2016). In vitro activity of quaternary ammonium surfactants against streptococcal, chlamydial, and gonococcal infective agents. Antimicrob Agents Chemother. 23;60(6):3323-32. doi: 10.1128/AAC.00166-16.

28.     M. Encarnação, L. Espada, C. Escrevente, D. Mateus, J. Ramalho, X. Michelet, I. Santarino, V. W. Hsu, M. B. Brenner, D. Barral and O. V. Vieira. (2016).* A Rab3a-dependent complex essential for lysosome positioning and plasma membrane repair. J. Cell Biol. 213 (6) 631-640.

* Spotlight in J. Cell Biol. 2016 213:613-615. It has. been recommended in F1000Prime.

29.    N. Domingues, L. Estronca, J. Silva, M. Encarnação, R. Mateus, D. Silva, I. Santarino, M. Saraiva, M. Soares, T. Pinho e Melo, A. Jacinto, W. Vaz and O.V. Vieira.(2016)  Cholesteryl hemiesters transform lysosome structure and induce proinflammatory cytokine production in macrophages. BBA - Molecular and Cell Biology of Lipids, accepted for publication.

30.    O.V. Vieira, R.J. Botelho, and S. Grinstein (2002). Phagosome maturation: Aging gracefully.

Biochem J. 366(Pt 3):689-704.

31.    A. Negre- Salvayre, O. Vieira, I. Escargueil-Blanc, and R. Salvayre (2003). Oxidized LDL and 4-hydroxynonenal modulate tyrosine kinase receptor activity. Mol. Aspects Med.24:251-261.

32.    M.L. Jordão and O.V. Vieira (2011). Tuberculosis: new aspects of an old disease.Int. J. Cell Biol.2011:403623.

33.    O.V. Vieira (2016). Rab3a and Rab10 are regulators of lysosome exocytosis and plasma membrane repair. Small GTPases. 2016 Sep 29:0. [Epub ahead of print]

9. INVITED PRESENTATIONS TO PEER-REVIEWED AND INTERNATIONALLY ESTABLISHED CONFERENCES (OF 21):

1. Vieira O.V. “Cholesteryl hemi-esters irreversible alter lysosome structure and function and are potent pro-inflammatory lipid species: implications for atherogenesis.” The Pathobiology of the Lysosome and Lysosomal Diseases Conference. July 2016. Cambridge, UK.

2. Winchil L. C. Vaz, Ângela S. Inácio and O.V. Vieira “Mechanisms of Action and Comparative Toxicity of Surfactants To Eukaryotic and Prokaryotic Cells In Vitro: Insights Into their Use as Antiseptic Agents”.

6th. Iberian Meeting on Colloids and Interfaces" Guimarães, July 2015. 

3. Vieira, O. “Cardiovascular Disease Predictors from Lipidomic Analysis-Translation from the Laboratory Bench to the Hospital Bedside”. MIT Portugal Annual Conference, June, 2015, New University of Lisbon, Lisbon, Portugal.

4. Vieira O. “From bench to bedside: the role of cholesteryl hemiesters in coronary artery disease”. 2VRT, Vascular and Valvular. Restorative Therapy, May, 2015.

5. Vieira O. “Membrane Traffic in host/pathogen interactions and in cholesterol homeostasis.” IGC, Oeiras, Portugal, 2013.

6-Vieira, O. “Mechanisms of Host Plasma Membrane Repair in Infection by M. Tuberculosis”. Workshop organised under the MTBSS EU/ India Cooperation Project. IGC, Oeiras, Portugal, 2012.

7- Vieira, O. et al. “Surfactants in the Prophylaxis of Sexually Transmitted Infections”. X International Symposium on Experimental Techniques and I Congress of the Society for Medical Research on Complementary Therapeutics. Coimbra, Portugal, 2010.

8-Vieira, O. “Regulation of phagosomal maturation by phosphatidylinositol 3-phosphate”. FEBS meeting, Lisbon, Portugal, 2001.

10. SCIENTIFIC NETWORKS

2012-2015: Coordinator of a consortium formed by my group and Harvard Medical School (HMS) laboratories. This program aims at promoting new inter-institutional Translational Medicine projects.

11. REVIEW PANELS

Ad-hoc reviewer for PLoS One, Langmuir, Environmental Science & Technology, BBA, etc.

12. GRADUATE TEACHING EXPERIENCE

2015: “Chronic Diseases” ProRegem, PhD Program, CEDOC, FCM, New University of Lisbon.

2013: Membrane Traffic and Disease,doctoral programme in Experimental Biology and Biomedicine, University of Coimbra, Portugal.

2012: “Cell Regulation”, Masters Program in Cellular and Molecular Biology, Department of Life Sciences, University of Coimbra.

2011-2013: “Molecular Basis of Disease”, Inter-University Doctoral Programme on Ageing and Degeneration of Complex Biological Systems, coordinated by the University of Coimbra, University of Lisbon, and University of Minho.

2009-2011: Member of the teaching staff of the Ph.D. − MIT/Portugal programme.

2008 and 2010: Cell Biology of Infection, doctoral programme in Experimental Biology and Biomedicine, University of Coimbra, Portugal.

2004-2005: Member of the teaching staff of PhD programme at the Max-Planck-Institute for Molecular Cell Biology and Genetics, Dresden, Germany.

2002: Teaching and optimization of phagosome maturation assays in macrophage-like Drosophila S2 cells in the laboratory of Dr. Alan Ezekowitz  (Lab. of Developmental Immunology, Massachusetts General Hospital for Children, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts , USA).

CAREER BREAKS:Maternity leave (18 months).