• FCT-NOVA, Campus Caparica, Unidade de Ciências Biomoleculares Aplicadas (UCIBIO)

    2829-516 Caparica

  • FCT-NOVA, Faculdade de Ciências e Tecnologia, Campus Caparica, Departamento de Química

    2829-516 Caparica

    Portugal

Accepting PhD Students

  • 5345 Citations
  • 39 h-Index
1993 …2019
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Research Output at NOVA 1993 2019

2019

Aldehyde Oxidases as Enzymes in Phase I Drug Metabolism

Mota, C., Santos-Silva, T. S., Terao, M., Garattini, E., Romão, M. J. & Leimkühler, S., 27 Jun 2019, Pharmaceutical Biocatalysis: Fundamentals, Enzyme Inhibitors, and Enzymes in Health and Diseases. Grunwald, P. (ed.). Routledge Taylor & Francis Group, 27 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Aldehyde Oxidase
Aldehyde Oxidoreductases
Xanthine Dehydrogenase
Enzymes
Pharmaceutical Preparations
3 Citations (Scopus)
1 Downloads (Pure)

Human aldehyde oxidase (hAOX1): structure determination of the Moco-free form of the natural variant G1269R and biophysical studies of single nucleotide polymorphisms

Mota, C., Esmaeeli, M., Coelho, C., Santos-Silva, T., Wolff, M., Foti, A., Leimkühler, S. & Romão, M. J., 1 May 2019, In : FEBS Open Bio. 9, 5, p. 925-934 10 p.

Research output: Contribution to journalArticle

Open Access
File
Aldehyde Oxidase
Polymorphism
Single Nucleotide Polymorphism
Nucleotides
Enzymes
2018
7 Citations (Scopus)

Critical overview on the structure and metabolism of human aldehyde oxidase and its role in pharmacokinetics

Mota, C., Coelho, C., Leimkühler, S., Garattini, E., Terao, M., Santos-Silva, T. & Romão, M. J., 1 Aug 2018, In : Coordination Chemistry Reviews. 368, p. 35-59 25 p.

Research output: Contribution to journalReview article

Aldehyde Oxidase
Pharmacokinetics
oxidase
metabolism
Aldehydes
7 Citations (Scopus)
22 Downloads (Pure)
Open Access
File
Peptidoglycan
Gram-Positive Bacteria
Glutamine
Staphylococcus aureus
Glutaminase
2 Citations (Scopus)

Irreversible Magnetic Behaviour Caused by the Thermosalient Phenomenon in an Iron(III) Spin Crossover Complex

Martins, F. F., Joseph, A., Diogo, H. P., Minas da Piedade, M. E., Ferreira, L. P., Carvalho, M. D., Barroso, S., Romão, M. J., Calhorda, M. J. & Martinho, P. N., 6 Jul 2018, In : European Journal of Inorganic Chemistry. 2018, 25, p. 2976-2983 8 p.

Research output: Contribution to journalArticle

Open Access
Hysteresis loops
Iron
Crystals
Hysteresis
Differential scanning calorimetry

Structural elucidation of macromolecules

Romão, M. J., Santos-Silva, T. S., Carvalho, A. L., Cabrita, E. J. & Marcelo, F. M. B. M., 2018, Essential Techniques for Medical and Life Scientists : A Guide to Contemporary Methods and Current Applications With the Protocols. Bentham Science Publishers Ltd., Vol. Pt.1. p. 30-91 61 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Macromolecules
Ligands
X ray crystallography
X ray scattering
Nucleic Acids

The crystal structure of human aldehyde oxidase

Romao, M. J., Jan 2018, In : DRUG METABOLISM AND PHARMACOKINETICS. 33, 1, p. S10-S11

Research output: Contribution to journalMeeting Abstract

4 Citations (Scopus)
6 Downloads (Pure)

The crystal structure of the R280K mutant of human p53 explains the loss of DNA binding

Gomes, A. S., Trovão, F., Andrade Pinheiro, B., Freire, F., Gomes, S., Oliveira, C., Domingues, L., Romão, M. J., Saraiva, L. & Carvalho, A. L., 13 Apr 2018, In : International Journal of Molecular Sciences. 19, 4, 1184.

Research output: Contribution to journalArticle

Open Access
File
DNA
deoxyribonucleic acid
Crystal structure
crystal structure
cancer
2017
4 Citations (Scopus)

A synthetic route to novel 3-substituted-2,1-benzisoxazoles from 5-(2-nitrobenzylidene)(thio)barbiturates

Serrano, J. L., Cavalheiro, E., Barroso, S., Romão, M. J., Silvestre, S. & Almeida, P., 1 Nov 2017, In : Comptes Rendus Chimie. 20, 11-12, p. 990-995 6 p.

Research output: Contribution to journalArticle

Barbiturates
Xanthine Oxidase
Isoxazoles
X ray analysis
Bioactivity
3 Citations (Scopus)

Electron transfer through arsenite oxidase: Insights into Rieske interaction with cytochrome c

Watson, C., Niks, D., Hille, R., Vieira, M., Schoepp-Cothenet, B., Marques, A. T., Romão, M. J., Santos-Silva, T. & Santini, J. M., 1 Oct 2017, In : Biochimica et Biophysica Acta - Bioenergetics. 1858, 10, p. 865-872 8 p.

Research output: Contribution to journalArticle

Cytochromes c
Electrons
Calorimetry
Biosensing Techniques
Titration
4 Citations (Scopus)
Crystallization
Self assembly
self assembly
crystallization
Infrared radiation
5 Citations (Scopus)

Ion-Exchange Membranes for Stable Derivatization of Protein Crystals

Polino, M., Carvalho, A. L., Juknaite, L., Portugal, C. A. M., Coelhoso, I. M., Romão, M. J. & Crespo, J. G., 6 Sep 2017, In : Crystal Growth and Design. 17, 9, p. 4563-4572 10 p.

Research output: Contribution to journalArticle

Ion exchange membranes
membranes
proteins
Proteins
Crystals
3 Citations (Scopus)

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C

Pires, V. M. R., Pereira, P. M., Brás, J. L. A., Correia, M., Cardoso, V., Bule, P., Alves, V. D., Najmudin, S., Venditto, I., Ferreira, L. M. A., Romão, M. J., Carvalho, A. L., Fontes, C. M. G. A. & Prazeres, D. M., 24 Mar 2017, In : Journal of Biological Chemistry. 292, 12, p. 4847-4860 14 p.

Research output: Contribution to journalArticle

Spirochaeta
Carbohydrates
Ligands
Cellulose
Plant Cells
11 Citations (Scopus)

Structural basis for the role of mammalian aldehyde oxidases in the metabolism of drugs and xenobiotics

Romão, M. J., Coelho, C., Santos-Silva, T., Foti, A., Terao, M., Garattini, E. & Leimkühler, S., 1 Apr 2017, In : Current Opinion in Chemical Biology. 37, p. 39-47 9 p.

Research output: Contribution to journalReview article

Aldehyde Oxidase
Xenobiotics
Metabolism
Pharmaceutical Preparations
Isoenzymes
2016

An integrative strategy to decipher glycan recognition in the human gut microbiome

Correia, V. G., Bras, J. L. A., Liu, Y., Silva, L., Zhang, Y., Pinheiro, B. A., Romao, M. J., Carvalho, A. L., Chai, W., Fontes, C. M. G. A., Feizi, T. & Palma, A. S., Dec 2016, In : Glycobiology. 26, 12, p. 1398-1399 2 p.

Research output: Contribution to journalMeeting Abstract

10 Citations (Scopus)
2 Downloads (Pure)

Diverse specificity of cellulosome attachment to the bacterial cell surface

Brás, J. L. A., Pinheiro, B. A., Cameron, K., Cuskin, F., Viegas, A., Najmudin, S., Bule, P., Pires, V. M. R., Romão, M. J., Bayer, E. A., Spencer, H. L., Smith, S. P., Gilbert, H. J., Alves, V. D., Carvalho, A. L. & Fontes, C. M. G. A., 7 Dec 2016, In : Scientific Reports. 6, p. 38292

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Anaerobic Bacteria
Proteins
Carbohydrates
cohesins
14 Citations (Scopus)

Optimization of the expression of human aldehyde oxidase for investigations of single-nucleotide polymorphisms

Foti, A., Hartmann, T., Coelho, C. M. C., Santos-Silva, T., Romão, M. J. & Leimkühler, S., 1 Aug 2016, In : Drug Metabolism And Disposition. 44, 8, p. 1277-1285 9 p.

Research output: Contribution to journalArticle

Aldehyde Oxidase
Single Nucleotide Polymorphism
Enzymes
Amino Acids
Codon
39 Citations (Scopus)

Structure and function of mammalian aldehyde oxidases

Terao, M., Romao, M. J., Leimkuehler, S., Bolis, M., Fratelli, M., Coelho, C. M. C., Santos-Silva, T. S. & Garattini, E., Apr 2016, In : Archives Of Toxicology. 90, 4, p. 753-780 28 p.

Research output: Contribution to journalLiterature review

Open Access
8 Citations (Scopus)

The Escherichia coli Periplasmic Aldehyde Oxidoreductase Is an Exceptional Member of the Xanthine Oxidase Family of Molybdoenzymes

Correia, M. A. S., Otrelo-Cardoso, A. R., Schwuchow, V., Sigfridsson Clauss, K. G. V., Haumann, M., Romão, M. J., Leimkühler, S. & Santos-Silva, T., 21 Oct 2016, In : ACS Chemical Biology. 11, 10, p. 2923-2935 13 p.

Research output: Contribution to journalArticle

Aldehyde Oxidoreductases
Xanthine Oxidase
Escherichia coli
Crystal structure
Gene transfer
2015
32 Citations (Scopus)

A contribution to the rational design of Ru(CO)3Cl2L complexes for in vivo delivery of CO

Seixas, J. D., Santos, M. F. A., Mukhopadhyay, A., Coelho, A. C., Reis, P. M., Veiros, L. F., Marques, A. R., Penacho, N., Gonçalves, A. M. L., Romão, M. J., Bernardes, G. J. L., Santos-Silva, T. S. & Romao, C. J., 21 Mar 2015, In : Dalton Transactions. 44, 11, p. 5058-5075 18 p.

Research output: Contribution to journalArticle

Cytotoxicity
Gases
Derivatives
Heme Oxygenase (Decyclizing)
Water gas shift
2 Citations (Scopus)
16 Citations (Scopus)

Insights into the structural determinants of substrate specificity and activity in mouse aldehyde oxidases

Cerqueira, N. M. F. S. A., Coelho, C. M. C., Brás, N. F., Fernandes, P. A., Garattini, E., Terao, M., Romão, M. J. & Ramos, M. J., 2015, In : Journal Of Biological Inorganic Chemistry. 20, 2, p. 209-217 9 p.

Research output: Contribution to journalArticle

Aldehyde Oxidase
Substrate Specificity
Protein Isoforms
Substrates
Binding Sites
5 Citations (Scopus)

Ionic-Liquid-Functionalized Mineral Particles for Protein Crystallization

Kowacz, M., Marchel, M., Juknaité, L., Esperança, J. M. S. S., Romão, M. J., Carvalho, A. L. & Rebelo, L. P. N., Jun 2015, In : Crystal Growth & Design. 15, 6, p. 2994-3003 10 p.

Research output: Contribution to journalArticle

Ionic Liquids
Crystallization
Ionic liquids
Minerals
minerals
16 Citations (Scopus)

Structural and mechanistic insights on nitrate reductases

Coelho, C. M. C. & Romão, M. J., 1 Dec 2015, In : Protein Science. 24, 12, p. 1901-1911 11 p.

Research output: Contribution to journalReview article

Nitrate Reductases
Crystal structure
Desulfovibrio desulfuricans
Nitrogen Cycle
Enzymes
45 Citations (Scopus)

Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase

Coelho, C. M. C., Foti, A., Hartmann, T., Santos-Silva, T. S., Leimkühler, S. & Romão, M. J., 2015, In : Nature Chemical Biology. 11, 10, p. 779-783 5 p.

Research output: Contribution to journalArticle

Aldehyde Oxidase
Xenobiotics
Thioridazine
Xanthine Oxidase
Catalytic Domain
2014
5 Citations (Scopus)

Biochemical, Stabilization and Crystallization Studies on a Molecular Chaperone (PaoD) Involved in the Maturation of Molybdoenzymes

Otrelo-Cardoso, A. R., Schwuchow, V., Rodrigues, D., Cabrita, E. J., Leimkuehler, S., Romao, M. J. & Santos-Silva, T., 31 Jan 2014, In : PlosOne. 9, 1, 9 p., e87295.

Research output: Contribution to journalArticle

Erratum: Use of gold nanoparticles as additives in protein crystallization (Crystal Growth and Design (2014) 14:1 (222-227) DOI:10.1021/cg4014398)

Ribeiro, D., Kulakova, A., Quaresma, P., Pereira, E., Bonifácio, C., Romão, M. J., Franco, R. & Carvalho, A. L., 5 Feb 2014, In : Crystal Growth & Design. 14, 2, p. 888 1 p.

Research output: Contribution to journalComment/debate

Open Access
6 Citations (Scopus)

Structural Data on the Periplasmic Aldehyde Oxidoreductase PaoABC from Escherichia coli: SAXS and Preliminary X-ray Crystallography Analysis

Otrelo-Cardoso, A. R., da Silva Correia, M. A., Schwuchow, V., Svergun, D. I., Romão, M. J., Leimkuehler, S. & Santos-silva, T. S., Feb 2014, In : International Journal of Molecular Sciences. 15, 2, p. 2223-2236

Research output: Contribution to journalArticle

Open Access
Aldehyde Oxidoreductases
X Ray Crystallography
X-Rays
Escherichia coli
Enzymes
12 Citations (Scopus)

Use of gold nanoparticles as additives in protein crystallization

Ribeiro, D., Kulakova, A., Quaresma, P., Pereira, E., Bonifácio, C., Romão, M. J., Franco, R. & Carvalho, A. L., 2 Jan 2014, In : Crystal Growth & Design. 14, 1, p. 222-227 6 p.

Research output: Contribution to journalArticle

Crystallization
Gold
crystallization
gold
Nanoparticles
2013
37 Citations (Scopus)

Characterization of a versatile organometallic pro-drug (CORM) for experimental CO based therapeutics

Seixas, J. D., Mukhopadhyay, A., Santos-silva, T. S., Otterbein, L. E., Gallo, D. J., Rodrigues, S. S., Guerreiro, B., Gonçalves, A. M. L., Penacho, N., Marques, A. R., Coelho, A. C., Reis, P. M., Romão, M. J. & Romao, C. C., 7 May 2013, In : Dalton Transactions. 42, 17, p. 5985-5998

Research output: Contribution to journalArticle

Prodrugs
Carbon Monoxide
Therapeutics
Animal Disease Models
Therapeutic Uses

Determinants of selective group reduction in the TNT-bound xenobiotic reductase B from P. putida

Carvalho, A. L. M. D. & Romão, M. J., 1 Jan 2013, 233 SPRING ST, NEW YORK, NY 10013 USA : Springer.

Research output: Other contribution

16 Citations (Scopus)

Identification of Crucial Amino Acids in Mouse Aldehyde Oxidase 3 That Determine Substrate Specificity

Romão, M. J., 1 Jan 2013, In : PLoS ONE. 8, 12, p. e82285

Research output: Contribution to journalArticle

Open Access
8 Citations (Scopus)
Nitrate Reductases
Electrochemistry
Heme
Peroxidase
Nitrates
48 Citations (Scopus)

Interaction of vanadium (IV) with human serum apo-transferrin.

Santos-silva, T. S. & Romão, M. J., 1 Jan 2013, In : Journal of Inorganic Biochemistry. 121, NA, p. 187-95

Research output: Contribution to journalArticle

Open Access
3 Citations (Scopus)
Open Access
File
Desulfovibrio gigas
Aldehyde Oxidoreductases
aldehyde oxidoreductases
enzyme activation
Enzyme Activation
13 Citations (Scopus)
Clostridium thermocellum
Carbohydrates
Ligands
Entropy
Proteins

Unraveling new functions and modes of action of molybdenum-dependent enzymes

Romão, M. J., 1 Jan 2013, 233 SPRING ST, NEW YORK, NY 10013 USA : Springer.

Research output: Other contribution

Vanadium compounds as prospective therapeutics: X-ray structure of protein adducts

Santos-silva, T. S. & Romão, M. J., 1 Jan 2013, 233 SPRING ST, NEW YORK, NY 10013 USA : Springer.

Research output: Other contribution

2012
4 Citations (Scopus)

Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes

Brás, J. L. A., Carvalho, A. L., Viegas, A., Najmudin, S., Alves, V. D., Prates, J. A. M., Ferreira, L. M. A., Romão, M. J., Gilbert, H. J. & Fontes, C. M. G. A., 22 May 2012, In : Methods in Enzymology. 510, p. 395-415 21 p.

Research output: Contribution to journalArticle

Cellulosomes
Bacterial Structures
Crystallization
Escherichia coli
Purification
27 Citations (Scopus)
Open Access
Ionic Liquids
Crystallization
Ionic liquids
crystallization
proteins
20 Citations (Scopus)

Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode

Brás, J. L. A., Alves, V. D., Carvalho, A. L., Najmudin, S., Prates, J. A. M., Ferreira, L. M. A., Bolam, D. N., Romão, M. J., Gilbert, H. J. & Fontes, C. M. G. A., 28 Dec 2012, In : Journal of Biological Chemistry. 287, 53, p. 44394-44405 12 p.

Research output: Contribution to journalArticle

Open Access
File
Clostridium thermocellum
Clostridium
Cellulosomes
Biological Phenomena
Cellulase
67 Citations (Scopus)
2 Downloads (Pure)

The first mammalian aldehyde oxidase crystal structure: Insights into substrate specificity

Coelho, C., Mahro, M., Trincão, J., Carvalho, A. T. P., Ramos, M. J., Terao, M., Garattini, E., Leimkühler, S. & Romão, M. J., 23 Nov 2012, In : Journal of Biological Chemistry. 287, 48, p. 40690-40702 13 p.

Research output: Contribution to journalArticle

Open Access
File
Aldehyde Oxidase
Substrate Specificity
Crystal structure
Substrates
Xanthine Dehydrogenase
2011
22 Citations (Scopus)
Open Access
121 Citations (Scopus)

CORM-3 Reactivity toward Proteins: The Crystal Structure of a Ru(II) Dicarbonyl-Lysozyme Complex

Santos-silva, T. S., Mukhopadhyay, A., Seixas, J. D., Bernardes, G. J. L., Romao, C. J. & Romão, M. J., 4 Jan 2011, In : Journal of the American Chemical Society. 133, 5, p. 1192-1195

Research output: Contribution to journalArticle

Open Access
Carbon Monoxide
Muramidase
Carbon monoxide
Enzymes
Crystal structure