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Research Output at NOVA 1990 2018

2018
3 Citations (Scopus)
8 Downloads (Pure)

A detection and quantification label-free tool to speed up downstream processing of model mucins

Carvalho, S. B., Moreira, A. S., Gomes, J., Carrondo, M. J. T., Thornton, D. J., Alves, P. M., Costa, J. & Peixoto, C., 1 Jan 2018, In : PLoS ONE. 13, 1, e0190974.

Research output: Contribution to journalArticle

Open Access
File
mucins
Mucins
Labels
lectins
Processing
2 Citations (Scopus)
1 Downloads (Pure)

Clinical-grade oncolytic adenovirus purification using polysorbate 20 as an alternative for cell lysis

Moleirinho, M. G., Rosa, S., Carrondo, M. J. T., Silva, R. J. S., Hagner-McWhirter, Å., Ahlén, G., Lundgren, M., Alves, P. M. & Peixoto, C., 1 Jan 2018, In : Current Gene Therapy. 18, 6, p. 366-374 9 p.

Research output: Contribution to journalArticle

Open Access
File
Polysorbates
Octoxynol
Adenoviridae
Clinical Trials, Phase I
Surface-Active Agents
7 Citations (Scopus)

Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production

Sequeira, D. P., Correia, R., Carrondo, M. J. T., Roldão, A., Teixeira, A. P. & Alves, P. M., 24 May 2018, In : Vaccine. 36, 22(SI), p. 3112-3123 12 p.

Research output: Contribution to journalArticle

virus-like particles
Baculoviridae
Hemagglutinins
hemagglutinins
Orthomyxoviridae
6 Citations (Scopus)
12 Downloads (Pure)

LentiPro26: Novel stable cell lines for constitutive lentiviral vector production

Tomás, H. A., Rodrigues, A. F., Carrondo, M. J. T. & Coroadinha, A. S., 1 Dec 2018, In : Scientific Reports. 8, 1, 5271.

Research output: Contribution to journalArticle

Open Access
File
Cell Line
Genetic Therapy
pol Gene Products
Clone Cells
Viral Structures
9 Citations (Scopus)

Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers

Carvalho, S. B., Fortuna, A. R., Wolff, M. W., Peixoto, C., Alves, P. M., Reichl, U. & Carrondo, M. J. T., 1 Jul 2018, In : Journal of Chemical Technology and Biotechnology. 93, 7, p. 1988-1996 9 p.

Research output: Contribution to journalArticle

virus particle
influenza
Orthomyxoviridae
Viruses
Cellulose
7 Citations (Scopus)

RMCE-based insect cell platform to produce membrane proteins captured on HIV-1 Gag virus-like particles

Vidigal, J. M. N., Fernandes, B., Dias, M. M., Patrone, M., Roldão, A., Carrondo, M. J. T., Alves, P. M. & Teixeira, A. P., 1 Jan 2018, In : Applied Microbiology and Biotechnology. 102, 2, p. 655-666 12 p.

Research output: Contribution to journalArticle

Recombinases
Virion
Insects
HIV-1
Membrane Proteins
2017
11 Citations (Scopus)

Bioprocess integration for human mesenchymal stem cells: From up to downstream processing scale-up to cell proteome characterization

Cunha, B., Aguiar, T., Carvalho, S. B., Silva, M. M., Gomes, R. A., Carrondo, M. J. T., Gomes-Alves, P., Peixoto, C., Serra, M. & Alves, P. M., 20 Apr 2017, In : Journal of Biotechnology. 248, p. 87-98 12 p.

Research output: Contribution to journalArticle

Proteome
Stem cells
Mesenchymal Stromal Cells
Proteins
Processing
2 Citations (Scopus)

Disclosing the Parameters Leading to High Productivity of Retroviral Producer Cells Lines: Evaluating Random Versus Targeted Integration

Bandeira, V. S., Tomás, H. A., Alici, E., Carrondo, M. J. T. & Coroadinha, A. S., 1 Apr 2017, In : Human Gene Therapy Methods. 28, 2, p. 78-90 13 p.

Research output: Contribution to journalArticle

Gibbon ape leukemia virus
Transgenes
Cell Line
Gammaretrovirus
env Genes
3 Citations (Scopus)

Finding the design space of a filtration-based operation for the concentration of human pluripotent stem cells

Cunha, B., Silva, R. J. S., Correia, C., Koshkin, A., Alves, P. M., Serra, M., Peixoto, C. & Carrondo, M. J. T., 1 Jan 2017, In : Journal of Membrane Science. 542, p. 399-407 9 p.

Research output: Contribution to journalArticle

Pluripotent Stem Cells
stem cells
Induced Pluripotent Stem Cells
Stem cells
Shear deformation
12 Citations (Scopus)

Universal label-free in-process quantification of influenza virus-like particles

Carvalho, S. B., Moleirinho, M. G., Wheatley, D., Welsh, J., Gantier, R., Alves, P. M., Peixoto, C. & Carrondo, M. J. T., 1 Aug 2017, In : Biotechnology Journal. 12, 8, 1700031.

Research output: Contribution to journalArticle

Orthomyxoviridae
Virion
Interferometry
Hemagglutinins
Technology
2016
8 Citations (Scopus)

Bioorthogonal Strategy for Bioprocessing of Specific-Site-Functionalized Enveloped Influenza-Virus-Like Particles

Carvalho, S. B., Freire, J. M., Moleirinho, M. G., Monteiro, F., Gaspar, D., Castanho, M. A. R. B., Carrondo, M. J. T., Alves, P. M., Bernardes, G. J. L. & Peixoto, C., 19 Oct 2016, In : Bioconjugate Chemistry. 27, 10, p. 2386-2399 14 p.

Research output: Contribution to journalArticle

Orthomyxoviridae
Viruses
Virion
Vaccines
Azides
8 Citations (Scopus)

Improving washing strategies of human mesenchymal stem cells using negative mode expanded bed chromatography

Cunha, B., Silva, R. J. S., Aguiar, T., Serra, M., Daicic, J., Maloisel, J. L., Clachan, J., Åkerblom, A., Carrondo, M. J. T., Peixoto, C. & Alves, P. M., 15 Jan 2016, In : Journal Of Chromatography A. 1429, p. 292-303 12 p.

Research output: Contribution to journalArticle

Chromatography
Stem cells
Mesenchymal Stromal Cells
Washing
Adsorption
8 Citations (Scopus)

In vitro expansion of human cardiac progenitor cells: Exploring 'omics tools for characterization of cell-based allogeneic products

Gomes-Alves, P., Serra, M., Brito, C., Ricardo, C. P., Cunha, J. R., Sousa, M. F., Teresa Blazquez-Sanchez, M., Bernad, A., Carrondo, M. J. T., Rodriguez-Borlado, L. & Alves, P. M., 1 May 2016, In : Translational Research. 171, p. 96-110 15 p.

Research output: Contribution to journalArticle

Stem cells
Stem Cells
Monolayers
Production platforms
Proteome
6 Citations (Scopus)
Canine Adenoviruses
Madin Darby Canine Kidney Cells
Glutamine
Metabolic Flux Analysis
Growth
9 Citations (Scopus)

Tetraspanins displayed in retrovirus-derived virus-like particles and their immunogenicity

Soares, H. R., Castro, R., Tomás, H. A., Rodrigues, A. F., Gomes-Alves, P., Bellier, B., Klatzmann, D., Carrondo, M. J. T., Alves, P. M. & Coroadinha, A. S., 18 Mar 2016, In : Vaccine. 34, 13, p. 1634-1641 8 p.

Research output: Contribution to journalArticle

Retroviridae
Tetraspanins
virus-like particles
Virion
immune response
2015
9 Citations (Scopus)

Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome

Gomes-Alves, P., Serra, M., Montes, A. C., R.-Borlado, L., López, J. A., Vázquez, J., Carrondo, M. J. T., Bernad, A. & Alves, P. M., 1 Apr 2015, In : Proteomics. 15, 7, p. 1332-1337 6 p.

Research output: Contribution to journalArticle

Cell membranes
Stem cells
Proteomics
Stem Cells
Cell Membrane
29 Citations (Scopus)

Exploring continuous and integrated strategies for the up- and downstream processing of human mesenchymal stem cells

Cunha, B., Aguiar, T., Silva, M. M., Silva, R. J. S., Sousa, M. F. Q., Pineda, E., Peixoto, C., Carrondo, M. J. T., Serra, M. & Alves, P. M., 10 Nov 2015, In : Journal of Biotechnology. 213, p. 97-108 12 p.

Research output: Contribution to journalArticle

Stem cells
Mesenchymal Stromal Cells
Processing
Cell death
Perfusion
21 Citations (Scopus)

Filtration methodologies for the clarification and concentration of human mesenchymal stem cells

Cunha, B., Peixoto, C., Silva, M. M., Carrondo, M. J. T., Serra, M. & Alves, P. M., 5 Mar 2015, In : Journal of Membrane Science. 478, p. 117-129 13 p.

Research output: Contribution to journalArticle

stem cells
Stem cells
Mesenchymal Stromal Cells
methodology
cells
7 Citations (Scopus)

Human amniocyte-derived cells are a promising cell host for adenoviral vector production under serum-free conditions

Silva, A. C., Simão, D., Küppers, C., Lucas, T., Sousa, M. F. Q., Cruz, P., Carrondo, M. J. T., Kochanek, S. & Alves, P. M., 1 May 2015, In : Biotechnology Journal. 10, 5, p. 760-771 12 p.

Research output: Contribution to journalArticle

Adenoviridae
Cell Count
Cell Line
Infection
Serum
44 Citations (Scopus)

Improved virus purification processes for vaccines and gene therapy

Nestola, P., Peixoto, C., Silva, R. R. J. S., Alves, P. M., Mota, J. P. B. & Carrondo, M. J. T., 1 May 2015, In : Biotechnology and Bioengineering. 112, 5, p. 843-857 15 p.

Research output: Contribution to journalReview article

Active Immunotherapy
Gene therapy
Vaccines
Viruses
Genetic Therapy

Novel flowthrough platform for viral vectors purification

Peixoto, C., Silva, R., Nestola, P., Villain, L., Mota, J. P. B. & Carrondo, M. J. T., 1 Oct 2015, In : Human Gene Therapy. 26, 10, p. A71-A71 1 p.

Research output: Contribution to journalMeeting Abstract

20 Citations (Scopus)

Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes

Sousa, M. F. Q., Silva, M. M., Giroux, D., Hashimura, Y., Wesselschmidt, R., Lee, B., Roldão, A., Carrondo, M. J. T., Alves, P. M. & Serra, M., 1 Nov 2015, In : Biotechnology Progress. 31, 6, p. 1600-1612 13 p.

Research output: Contribution to journalArticle

Human Adenoviruses
Bioreactors
Mesenchymal Stromal Cells
Cell Culture Techniques
Cell- and Tissue-Based Therapy
7 Citations (Scopus)
Viruses
Adenoviridae
Virion
Purification
Membranes
14 Citations (Scopus)

Robust design of adenovirus purification by two-column, simulated moving-bed, size-exclusion chromatography

Nestola, P., Silva, R. J. S., Peixoto, C., Alves, P. M., Carrondo, M. J. T. & Mota, J. P. B., 10 Nov 2015, In : Journal of Biotechnology. 213, p. 109-119 11 p.

Research output: Contribution to journalArticle

Hospital Bed Capacity
Size exclusion chromatography
Distillation columns
Adenoviridae
Uncertainty
2014
26 Citations (Scopus)

Adenovirus purification by two-column, size-exclusion, simulated countercurrent chromatography

Nestola, P., Silva, R. J. S., Peixoto, C., Alves, P. M., Carrondo, M. J. T. & Mota, J. P. B., 20 Jun 2014, In : Journal Of Chromatography A. 1347, p. 111-121 11 p.

Research output: Contribution to journalArticle

Countercurrent Distribution
Distillation columns
Chromatography
Adenoviridae
Purification
13 Citations (Scopus)

Cellular targets for improved manufacturing of virus-based biopharmaceuticals in animal cells

Rodrigues, A. F., Carrondo, M. J. T., Alves, P. M. & Coroadinha, A. S., 1 Dec 2014, In : Trends In Biotechnology. 32, 12, p. 602-607 6 p.

Research output: Contribution to journalReview article

Viruses
Cell culture
Animals
Cell Culture Techniques
Cells
30 Citations (Scopus)

Combining Hypoxia and Bioreactor Hydrodynamics Boosts Induced Pluripotent Stem Cell Differentiation Towards Cardiomyocytes

Correia, C., Serra, M., Espinha, N., Sousa, M., Montes, A. C., Burkert, K., Zheng, Y., Hescheler, J., Carrondo, M. J. T., Šarić, T. & Alves, P. M., 2014, In : Stem Cell Reviews and Reports. 10, 6, p. 786-801 16 p.

Research output: Contribution to journalArticle

Induced Pluripotent Stem Cells
Bioreactors
Hydrodynamics
Cardiac Myocytes
Cell Differentiation
10 Citations (Scopus)

Evaluation of novel large cut-off ultrafiltration membranes for adenovirus serotype 5 (Ad5) concentration

Nestola, P., Martins, D. L., Peixoto, C., Roederstein, S., Schleuss, T., Alves, P. M., Mota, J. P. B. & Carrondo, M. J. T., 29 Dec 2014, In : PlosOne. 9, 12, e115802.

Research output: Contribution to journalArticle

Ultrafiltration
Adenoviridae
ultrafiltration
Viruses
serotypes
12 Citations (Scopus)

Impact of grafting on the design of new membrane adsorbers for adenovirus purification

Nestola, P., Villain, L., Peixoto, C., Martins, D. L., Alves, P. M., Carrondo, M. J. T. & Mota, J. P. B., 10 Jul 2014, In : Journal of Biotechnology. 181, p. 1-11

Research output: Contribution to journalArticle

Open Access
Adenoviridae
Purification
Membranes
Viruses
Hydrogel
3 Citations (Scopus)

Industrial Large Scale of Suspension Culture of Insect Cells

Roldão, A., Cox, M., Alves, P., Carrondo, M. & Vicente, T., 4 Aug 2014, Industrial Scale Suspension Culture of Living Cells. Wiley/Blackwell, p. 348-389 42 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Insects
Suspensions
Cell Culture Techniques
Cells
Genes
9 Citations (Scopus)
Open Access
27 Citations (Scopus)

Metabolic responses of CHO cells to limitation of key amino acids

Duarte, T. M., Carinhas, N., Barreiro, L. C., Carrondo, M. J. T., Alves, P. M. & Teixeira, A. P., Oct 2014, In : Biotechnology and Bioengineering. 111, 10, p. 2095-2106 12 p.

Research output: Contribution to journalArticle

Asparagine
Cricetulus
Glutamate-Ammonia Ligase
Metabolism
Amino acids

Production of complex virus-like particles in insect cells

Fernandes, F., Teixeira, A. P., Carinhas, N., Carrondo, M. J. T. & Alves, P. M., 1 Jan 2014, Virus-like Particles in Vaccine Development. FUTURE MEDICINE LTD, p. 120-134 15 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Virion
Insects
Vaccines
Genetic Engineering
Systems Biology
8 Citations (Scopus)

Testing a new formulation for Peste des Petits Ruminants vaccine in Ethiopia

Silva, A. C., Yami, M., Libeau, G., Carrondo, M. J. T. & Alves, P. M., 19 May 2014, In : Vaccine. 32, 24, p. 2878-2881 4 p.

Research output: Contribution to journalArticle

Peste-des-Petits-Ruminants
peste des petits ruminants
Ethiopia
Vaccines
vaccines
31 Citations (Scopus)

Towards real-time monitoring of therapeutic protein quality in mammalian cell processes

Pais, D. A. M., Carrondo, M. J. T., Alves, P. M. & Teixeira, A. M., 2014, In : Current Opinion in Biotechnology. 30, p. 161-167 7 p.

Research output: Contribution to journalReview article

Cells
Proteins
Monitoring
Technology
Post Translational Protein Processing
2013
6 Citations (Scopus)
Open Access
46 Citations (Scopus)

Insect cells as production platform of complex virus-like particles

Fernandes, F., Teixeira, A. P., Carinhas, N., Carrondo, M. J. T. & Alves, P. M., 1 Jan 2013, In : Expert Review of Vaccines. 12, 2, p. 225-236

Research output: Contribution to journalArticle

Open Access
Virion
Insects
Viral Structural Proteins
Baculoviridae
Viral Genome
46 Citations (Scopus)

Metabolic signatures of GS-CHO cell clones associated with butyrate treatment and culture phase transition

Carinhas, N., Duarte, T. M., Barreiro, L. C., Carrondo, M. J. T., Alves, P. M. & Teixeira, A. P., 1 Dec 2013, In : Biotechnology and Bioengineering. 110, 12, p. 3244–3257

Research output: Contribution to journalArticle

Open Access
Clone cells
Glutamate-Ammonia Ligase
Butyrates
Phase Transition
Cricetulus
25 Citations (Scopus)
Open Access
25 Citations (Scopus)
Open Access
101 Citations (Scopus)
Open Access
12 Citations (Scopus)
Open Access
23 Citations (Scopus)
Open Access
27 Citations (Scopus)
Open Access
Cell Communication
Proteomics
2011
6 Citations (Scopus)
Open Access
2 Citations (Scopus)

Extracellular Purine and Pyrimidine Catabolism in Cell Culture

Carrondo, M., 1 Jan 2011, In : Biotechnology Progress. 27, 5, p. 1373-1382

Research output: Contribution to journalArticle

Open Access
7 Citations (Scopus)
Open Access