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Personal profile

Research interests

Upstream and downstream processes with physico – mathematical tools towards the development of bioprocesses for complex and novel biopharmaceuticals. Simplicity, feasibility, transferability and economics are criteria used to design processes that may find industrialization opportunities. Such criteria, design and process applications have been extended to vaccines (e.g. multivalent Influenza VLPs, retro-VLPs, HIG Gag-VLPs, ZIKV-VLPs, viral vaccines…) and viral vectors for gene therapy (e.g. adenoviruses, retroviruses, lentiviruses, AAVs,…), drawing from the expertise and research achievements of the other labs in the ACTU.

Optimization of mammalian and insect cells expression systems (stable or transient expression), evaluation of different bioreactor operation modes (e.g. fed-batch and perfusion), application of scale-down models for high-throughput screening, design of novel continuous purification processes, modelling towards biological and chemical process control, and scale-up for pre-clinical applications. More recently, metabolic systems biology (e.g. 13C tracer experiments for fluxome estimation and exometabolome profiling by 1H-NMR) has been assisting bioprocess development in areas such as cancer cell metabolism (e.g. lung and breast), brain cell metabolism in normal and pathological conditions, stem cell metabolism along cardiac and neural differentiation, and cell metabolic response to virus infection.

Keywords: Biopharmaceuticals, Vaccines, Virus, Proteins, Stem cells, Gene Therapy, Engineering, Process Development, GMP

Fingerprint Dive into the research topics where Manuel José Teixeira Carrondo is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 7 Similar Profiles
Virion Medicine & Life Sciences
Viruses Chemical Compounds
Baculoviridae Medicine & Life Sciences
Stem cells Chemical Compounds
Adenoviridae Medicine & Life Sciences
Purification Chemical Compounds
Insects Medicine & Life Sciences
Bioreactors Medicine & Life Sciences

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Research Output at NOVA 1990 2018

3 Citations (Scopus)
2 Downloads (Pure)

A detection and quantification label-free tool to speed up downstream processing of model mucins

Carvalho, S. B., Moreira, A. S., Gomes, J., Carrondo, M. J. T., Thornton, D. J., Alves, P. M., Costa, J. & Peixoto, C., 1 Jan 2018, In : PLoS ONE. 13, 1, e0190974.

Research output: Contribution to journalArticle

Open Access
File
mucins
Mucins
Labels
lectins
Processing
1 Downloads (Pure)

Clinical-grade oncolytic adenovirus purification using polysorbate 20 as an alternative for cell lysis

Moleirinho, M. G., Rosa, S., Carrondo, M. J. T., Silva, R. J. S., Hagner-McWhirter, Å., Ahlén, G., Lundgren, M., Alves, P. M. & Peixoto, C., 1 Jan 2018, In : Current Gene Therapy. 18, 6, p. 366-374 9 p.

Research output: Contribution to journalArticle

Open Access
File
Polysorbates
Octoxynol
Adenoviridae
Clinical Trials, Phase I
Surface-Active Agents
6 Citations (Scopus)

Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production

Sequeira, D. P., Correia, R., Carrondo, M. J. T., Roldão, A., Teixeira, A. P. & Alves, P. M., 24 May 2018, In : Vaccine. 36, 22(SI), p. 3112-3123 12 p.

Research output: Contribution to journalArticle

virus-like particles
Baculoviridae
Hemagglutinins
hemagglutinins
Orthomyxoviridae
3 Citations (Scopus)
8 Downloads (Pure)

LentiPro26: Novel stable cell lines for constitutive lentiviral vector production

Tomás, H. A., Rodrigues, A. F., Carrondo, M. J. T. & Coroadinha, A. S., 1 Dec 2018, In : Scientific Reports. 8, 1, 5271.

Research output: Contribution to journalArticle

Open Access
File
Cell Line
Genetic Therapy
pol Gene Products
Clone Cells
Viral Structures
6 Citations (Scopus)

Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers

Carvalho, S. B., Fortuna, A. R., Wolff, M. W., Peixoto, C., Alves, P. M., Reichl, U. & Carrondo, M. J. T., 1 Jul 2018, In : Journal of Chemical Technology and Biotechnology. 93, 7, p. 1988-1996 9 p.

Research output: Contribution to journalArticle

virus particle
influenza
Orthomyxoviridae
Viruses
Cellulose