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Research interests

TEACHING EXPERIENCE General Biochemistry, Analytical and Inorganic Chemistry, Nuclear and Electron Magnetic Resonance and Mossbauer Spectroscopy (applications to Chemistry and Biology). Undergraduate, Master and PhD programs. 
RESEARCH ACTIVITY
Key words Bioinorganic, Biophysics, Biocatalysis, Energy Bioconversion (Hydrogen) Role of metals in Biology (heme and non-heme iron, molybdenum, tungsten, nickel , vanadium and cobalt) Inorganic systems as models for biocatalysis Spectroscopy (NMR, EPR and Mössbauer), (Bio) Electrochemistry 
Main goal is the understanding of electron transfer chains (ETC) of sulphate reducing bacteria (SRB) and methane forming bacteria (MFB). SRB have complex ETC systems which allow the reduction of sulphate by oxidation of either organic compounds or molecular hydrogen. The result of the bacterial activity results in the formation of large amounts of sulphide which presents serious health and environmental problems (metal corrosion, poisoning, etc). Another interest of these microorganisms is due to the fact that can either use or produce molecular hydrogen. In this last case, hydrogen is oxidized by other microbial groups (MFB) producing methane by reduction of carbon dioxide. The understanding of the involved mechanisms is important to study the use of new forms of energy (bioconversion - biological production of hydrogen and methane). A necessary step is the identification and isolation of metalloenzymes involved. The main goal is the use of spectroscopic techniques namely electron paramagnetic resonance (RPE) and related methods (ENDOR and ESEEM), uni- and multi-dimensional nuclear magnetic resonance RMN) and Mossbauer spectroscopy on the study of structure-function relationship of metallo-proteins and reconstitution of electron transfer chains (protein/protein interactions using Molecular graphics). The active centers that have been studied include iron-sulphur clusters (rubredoxin type, [2Fe -2S], [3Fe-4S], [4Fe-4S] and novel Fe-S centers), as well as associations with molybdenum and nickel) and hemes. Special relevance has been given to the characterization of bacterial hydrogenases (the role of the nickel in hydrogen evolution/ consumption) and to the topics: engineering of iron-sulphur proteins, chemical modification of active centers, synthesis of novel metal clusters (formation of clusters containing hetero-atoms) and molecular modeling. Research highlights: Discovery of a novel 3Fe center and Heterometallic Fe-S sites Isolation of novel Metalloproteins Role of Nickel in Hydrogenases Role of Molybdenum (and Tungsten) in Biology Electron Transfer and Protein-Protein Interactions in Metalloproteins

Fingerprint Dive into the research topics where José João Galhardas de Moura is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

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Desulfovibrio gigas Medicine & Life Sciences
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Paramagnetic resonance Chemical Compounds
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Heme Chemical Compounds
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Rubredoxins Chemical Compounds
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Research Output at NOVA 1982 2019

2 Citations (Scopus)
formic acid
Formate Dehydrogenases
Molybdenum
Carbon Dioxide
Desulfovibrio desulfuricans
1 Citation (Scopus)
Marinobacter
Nitric oxide
nitric oxide
Nitric Oxide
Oxygen
Open Access
Cytochromes b5
Heme
Ionization
Tyrosine
Ligands

Molybdenum and tungsten enzymes redox properties – A brief overview

Cordas, C. M. & Moura, J. J. G., 1 Sep 2019, In : Coordination Chemistry Reviews. 394, p. 53-64 12 p.

Research output: Contribution to journalReview article

Tungsten
Molybdenum
molybdenum
enzymes
tungsten
Nitration
Sulfites
Nitrites
Tyrosine
Proteins