Education

Apil 2017 – Present     FACULDADE DE CIÊNCIAS E TECNOLOGIA

                                     UNIVERSIDADE NOVA DE LISBOA (FCT-UNL)

Laboratory of Nanomedicine, DCV, FCT-UNL, Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/);

                                     CENIMAT/I3N, FCT-UNL, Monte da Caparica, Portugal

Postdoctorate Research Fellow - Flexible Ion Sensitive Sensors for Isothermal Gene Expression Analysis.

Jan 2012 – Jul 2016    FACULDADE DE CIÊNCIAS E TECNOLOGIA

                                     UNIVERSIDADE NOVA DE LISBOA (FCT-UNL)

Laboratory of Eukaryotic Molecular Genetics, DCV, FCT-UNL, Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/);

                                     CENIMAT/I3N, FCT-UNL, Monte da Caparica, Portugal

Doctorate Degree in Biotechnology - Development of Field Effect sensors for gene expression analysis: Application to cancer diagnosis.

Sep 2007 – Out 2009  FACULDADE DE CIÊNCIAS E TECNOLOGIA

                                     UNIVERSIDADE NOVA DE LISBOA (FCT-UNL)

                                     Monte da Caparica, Portugal

                                     (http://www.fct.unl.pt)

Master Degree in Biotechnology – Au-nanoprobes for the detection of Mycobacterium tuberculosis and Plasmodium berghei

Sep 1999 – Sep 2007   FACULDADE DE CIÊNCIAS E TECNOLOGIA

                                     UNIVERSIDADE NOVA DE LISBOA (FCT-UNL)

                                     Monte da Caparica, Portugal

                                     (http://www.fct.unl.pt)

Degree in Applied Chemistry – Biotechnology

Sep 1996 – Sep 1999   ESCOLA SECUNDÁRIA REYNALDO DOS SANTOS

                                     Vila Franca de Xira, Portugal

                                     Scientific/Natural Sciences Group - General

Professional Experience

Apr 2020 -Present       AlmaScience -Beyond Paper

(http://www.almascience.pt /);

Senior Researcher -Biosensors

Mar 2017 -Mar 2020    FACULDADE DE CIÊNCIAS E TECNOLOGIA

                                     UNIVERSIDADE NOVA DE LISBOA (FCT-UNL)

Laboratory of Nanomdicine, DCV, FCT-UNL, Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/);

Pos-Doc Research Grant (Research Grant Funded byFCT/MCES)

-       Pursuing research for Flexible Ion Sensitive Sensors for Isothermal Gene Expression Analysis.

Oct 2016 – Mar 2017   FACULDADE DE CIÊNCIAS E TECNOLOGIA

                                     UNIVERSIDADE NOVA DE LISBOA (FCT-UNL)

Laboratory of Nanomdicine, DCV, FCT-UNL, Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/);

Pos-Doc Research Grant (Research Grant Funded by INNO INDIGO ERA-Net Project

-       Pursuing research for one platform-multiple biomarker detection of Rheumatoid Arthritis.

Aug 2010 – Dec 2012 Laboratory of Eukaryotic Molecular Genetics, DCV, FCT-UNL

                                     Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/)

                                     Researcher (Research Grant Funded by FCT/MCES)

-       Pursuing research in Nanodiagnostics: gold-nanoprobes for molecular recognition - application to DNA adducts detection; pathogen detection; gene expression and RNA quantification

Out 2009 – Aug 2010 Laboratory of Eukaryotic Molecular Genetics, DCV, FCT-UNL

                                     Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/)

                                     Researcher (Research Grant Funded by FCT/MCES)

-       Pursuing research in nanotechnology: gold nanoparticles for detection of SNPs associated with antibiotic resistance in Mycobacterium tuberculosis

-       Pursuing research towards a evaluation and validation of an amorphous silicon based bio-optoelectronic portable platform, integrated with a DNA detection method based on Gold nanoprobes

Sep 2006 – Sep 2007  Laboratory of Eukaryotic Molecular Genetics, DCV, FCT-UNL

                                     Monte da Caparica, Portugal (http://pessoa.fct.unl.pt/pmvb/)

                                     Lab Technician (Internship )

- Pursuing research in nanotechnology: gold nanoparticles for the labelling of DNA probes towards the specific identification of nucleic acids

Publications

Papers (25)

“Water safety screening via multiplex LAMP-Au-nanoprobe integrated approach”, Beatriz B Oliveira, Bruno Veigas, Fábio Ferreira Carlos, Alexandre Sánchez-Melsió, José Luís Balcázar, Carles M. Borrego, Pedro Viana Baptista. 2020. Science of the Total Environment (in press).

“Fast Prototyping Microfluidics: Integrating Droplet Digital Lamp for Absolute Quantification of Cancer Biomarkers” Oliveira B, Veigas B, Fernandes AR, Águas H, Martins R, Fortunato E, Baptista PV. 2020. Sensors. DOI: 10.3390/s20061624.

“Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk” Ana Marques, Bruno Veigas, Andreia Araújo, Beatriz Pagará, Pedro Viana Baptista, Hugo Águas, Rodrigo Martins & Elvira Fortunato. 2019. Scientific Reports. 9, 17922.

“Antibody Modified Gold Nanoparticles for Fast Colorimetric screening of Rheumatoid arthritis” Bruno Veigas Ana Matias, Tomás Calmeiro, Elvira Fortunato, Alexandra R. Fernandes, Pedro Viana Baptista. 2019. Analyst. DOI: 10.1039/C9AN00319C

“GLUT1 and GLUT3 involvement in anthocyanin gastric transport-Nanobased targeted approach” Oliveira H., Roma-Rodrigues C., Santos A., Veigas B., Faria A., Calhau C., Freitas V., Fernandes A.R., Baptista P.V., Nuno Mateus & Fernandes I. 2019. Scientific Reports.  9:789.

“A Digital Microfluidics Platform for Loop-Mediated Isothermal Amplification Detection” Beatriz Jorge Coelho, Bruno Veigas, Hugo Águas, Elvira Fortunato, Rodrigo Martins, Pedro Viana Baptista, Rui Igreja. 2017. Sensors. 17(11).

“Allele specific LAMP- gold nanoparticle for characterization of single nucleotide polymorphisms” Fábio Carlos, Bruno Veigas, Ana Matias, Gonçalo Doria, Orfeu Flores, Pedro Baptista. 2017. Biotechnology Reports. BTRE_2017_166_R2.

 “Digital Microfluidics for Nucleic Acid Amplification”, Coelho B, Veigas B, Fortunato E, Martins R, Águas H, Igreja R, Baptista PV. 2017. Sensors. 17(7), 1495.

“Quantitative real-time monitoring of RCA amplification of cancer biomarkers mediated by a flexible ion sensitive platform”, Veigas B, Pinto J, Vinhas R, Calmeiro T, Martins R, Fortunato E, Baptista PV. 2017. Biosens Bioelectron. 91:788-795.

Scalable approach for the production of functional DNA based Gold nanoprobes”, Bruno Veigas, Carla Portugal, Rita Valério, J. P. Crespo, Pedro V. Baptista, 2015. Journal of Membrane Science. 492:528-535.

“Field Effect sensors for nucleic acid detection: Recent advances and future perspectives”, Bruno Veigas, Elvira Fortunato, Pedro V. Baptista, 2015. Sensors. 15(5):10380-10398.   

“One nanoprobe, two pathogens: gold nanoprobes multiplexing for point-of-care”,

Bruno Veigas, Pedro Pedrosa, Fábio F. Carlos, Liliana Mancio-Silva, Ana Rita Grosso, Elvira Fortunato, Maria M. Mota, Pedro V. Baptista, 2015. Journal of NanoBiotechnology. 13:48.

“Single nucleotide polymorphism detection using gold nanoprobes and bio-microfluidic platform with embedded microlenses”, Bernacka-Wojcik I, Águas H, Carlos FF, Lopes P, Wojcik PJ, Costa MN, Veigas B, Igreja R, Fortunato E, Baptista PV, Martins R, 2015. Biotechnol Bioeng. 112(6):1210-1219. 

“Gold nanoprobes for multi loci assessment of Multi-drug Resistant Tuberculosis“, Pedrosa P, Veigas B, Machado D, Couto I, Viveiros M, Baptista PV, 2014. Tuberculosis. 94 332-337.

“Low cost, safe, disposable, rapid and self-sustainable paper-based platform for diagnostic testing: Lab-on-Paper“,Costa M, Veigas B, Jacob J, Santos D, Gomes J, Baptista PV, Martins R, Inácio J, Fortunato E, 2014. Nanotechnology. 25(9):094006

“Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis”, Veigas B, Pedrosa P, Couto I, Viveiros M, Baptista PV, 2013. Analytical and Bioanalytical Chemistry. 11:38.

“Ion sensing (EIS) real-time quantitative monitorization of isothermal DNA amplification”, Veigas B, Branquinho R, Pinto JV, Wojcikb PJ, Martins R, Fortunato E, Baptista PV, 2013. Biosensors and Bioelectronics. 52:50-55.

“Bio-microfluidic platform for gold nanoprobe based DNA detection-application to Mycobacterium tuberculosis”, Bernacka-Wojcik I, Lopes P, Vaz AC, Veigas B, Wojcik PJ, Simões P, Barata D, Fortunato E, Baptista PV, Aguas H, Martins R, 2013. Biosensors and Bioelectronics, 48, 87-93.

“Gold on Paper – Paper platform for Au-nanoprobe TB detection”, Veigas B, Jacob J, Costa M, Santos D, Viveiros M, Inácio J, Martins R, Barquinha P, Fortunato E and Baptista PV, 2012. Lab on Chip. 12(22):4802-4808.

“Nanoprobes for TB diagnostics”, Baptista PV, Veigas B, Portugal I, Couto I, Viveiros M, 2012. Microbiologia 1(1). Portuguese Society for Microbiology Magazine.

“Contribution of efflux to the emergence of isoniazid and multidrug resistance in Mycobacterium tuberculosis”, Machado D, Couto I, Perdigão J,  Rodrigues L, Portugal I, Baptista PV, Veigas B, Amaral L, Viveiros M, 2012. PLoS ONE 7(4): e34538.

“Noble Metal Nanoparticles for Biosensing Applications”, Doria G, Conde J, Veigas B, Giestas L, Almeida C, Assunção M, Rosa J, Baptista PV, 2012. Sensors. 12(2):1657-1687.

“Real-time monitoring of PCR amplification of proto-oncogene c-MYC using a Ta₂O₅ electrolyte-insulator-semiconductor sensor”, Branquinho R, Veigas B, Pinto JV, Martins R, Fortunato E and Baptista PV, 2011, Biosensors and Bioelectronics, 28, 44–49.

“Portable optoelectronic biosensing platform for identification of mycobacteria from the Mycobacterium tuberculosis complex”, Silva LB, Veigas B, Doria G, Costa P, Inácio J, Martins R, Fortunato E and Baptista PV, 2010. Biosensors and Bioelectronics.(in Press: doi:10.1016/j.bios.2010.08.078).

“Au-nanoprobes for detection of SNPs associated with antibiotic resistance in Mycobacterium tuberculosis”, Veigas B, Machado D, Perdigão J, Portugal I, Couto I, Viveiros M, Baptista PV, 2010. Nanotechnology. (doi:10.1088/0957-4484/21/41/415101).

Chapters in Books (4)

“Mobile Based Gold Nanoprobe TB Diagnostics for Point-of-Need”, Veigas B., Fortunato E., Baptista PV. In “Mobile Health Technologies - Methods and Protocols” (Avraham Rasooly, Keith E. Herold). 2015. Springer (Humana Press), NY. pp. 41-56.

“AuNPs for identification of molecular signatures of resistance”, Bruno Veigas, Alexandra R. Fernandes, and PedroV.Baptista. doi: 10.3389/fmicb.2014.00455 Editor Marta Martins. 2014. Frontiers in Microbiology. 5:455.

“Gold Nanoparticles for DNA/RNA based diagnostics – Handbook of Gold nanoparticle research”, Ricardo Franco, Pedro Pedrosa, Fábio Carlos, Bruno Veigas and Pedro V. Baptista, 2013. Springer International Publishing. Chapter 50, pp.1339-1364.

“Nanodiagnostics for tuberculosis. Mycobacterium Tuberculosis / Book 1”, Gonçalo Doria, Bruno Veigas and Pedro V. Baptista. ISBN 979-953-307-078-9. Editor Pere-Joan Cardona, 2011. InTech – Open Access Publishers

Thesis (2)

“Development of Field Effect sensors for gene expression analysis: Application to cancer diagnosis”

Doctorate Thesis in Biotechnology, 2016.

"Au-nanoprobes for the detection of Mycobacterium tuberculosis and Plasmodium berghei" Master Thesis in Biotechnology, 2009.

Communications (Oral) (7)

B. J. Coelho, B Veigas, H Águas, E Fortunato, R Martins, PV Baptista, R Igreja. Digital Microfluidics Devices for Nucleic Acid Amplification: Redifining the Paradigm of Molecular Diagnostics;

In MATERIAIS 2017: VIII International Symposium on Materials

May 2017, Aveiro, Portugal

R. Martins, L. Pereira, A. Vicente, P. Barquinha, H. Águas, D. Gaspar, B. Veigas, MN Mafalda, T. Mateus, A. Araújo, Elvira Fortunato. Paper electronics, the breakthrough of the future;

Scientific Conference, Doctoral Schools of “Dunărea de Jos” 2015

June 2015,Galaţi, Romania

I. Bernacka-Wojcik, H. Aguas, P. Lopes, B. Veigas, P.J. Wojcik, P. Simões, R. Igreja, E. Fortunato, P.V. Baptista, R. Martins. Bio-Microfluidic platform for gold nanoprobe based DNA detection with embedded microlenses for improved performance;

Biosensors and Bioelectronics 2014

May 2014, Melbourne, Australia

Pedrosa P, Veigas B, Machado D, Perdigão J, Portugal I, Couto I, Viveiros M, Baptista PV. Detecção de MDRTB por gold-nanoprobes – Uma nova abordagem tecnológica desenvolvida em Portugal;

Workshop Multidrug-Resistant Tuberculosis.

April 2013, IHMT, Lisbon - Portugal

Branquinho R, Veigas B, Pinto JV, Martins R, Baptista PV, and Fortunato E; 2011.  Sputtered Ta₂O₅ Field Effect Based Biosensors;

EMRS 2011 Fall Meeting,

19-23 September 2011, Warsaw, Poland.

Viveiros, Miguel; Veigas, Bruno; Machado, Diana; Perdigão, João; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro; 2011. Direct detection of resistance in Mycobacterium tuberculosis: from line to nano-probe assays;

World TB Day,

22-11 Mar., Antwerp, Belgium.

Veigas, Bruno; Baptista, Pedro; Gold nanoparticles for low cost tuberculosis diagnostics;

Millennium Development Goals Conference,

March 2011, Caparica - Portugal

Communications (Posters) (9)

Coelho B. J., Veigas B., Águas H., Fortunato E., Martins R., Baptista P. V. & Igreja R. "Digital Microfluidics Devices for Nucleic Acid Amplification."

Biosensors, Miami (USA), 2018.

Coelho, B. J., Veigas, B., Águas, H., Fortunato, E., Martins, R., Baptista, P. V. & Igreja, R. "A Digital Microfluidics Platform for Loop-mediated Isothermal Amplification of DNA."

FEMS Junior EuroMAT, Budapest (Hungary), 2018.

B. Veigas, R. Branquinho, J.V. Pinto, P.J. Wojcik, R. Martins, E. Fortunato, P.V. Baptista.  Ion sensing (EIS) real-time quantitative monitorization of isothermal DNA amplification;

Biosensors and Bioelectronics 2014

May 2014, Melbourne, Australia

M.N. Costa, B. Veigas, J.M. Jacob, D.S. Santos, J. Gomes, P.V. Baptista, R. Martins, J. Inácio, E. Fortunato. A low cost, safe, disposable, rapid and self-sustainable-based paper platform for diagnostic testing, lab on paper;

Biosensors and Bioelectronics 2014

May 2014, Melbourne, Australia

Veigas B, Jacob J, Costa M, Santos D, Viveiros M, Inácio J, Martins R, Barquinha P, Fortunato E and Baptista PV; 2013. Gold on Paper – Paper platform for Au-nanoprobe TB detection;

NanoPortugal 2013.

13-15 February, 2013, Porto  - Portugal

Pedrosa P, Veigas B, Machado D, Perdigão J, Portugal I, Couto I, Viveiros M, Baptista PV, 2013, Gold nanoprobes methodology for diagnosis of Multi-Drug Resistant Tuberculosis;

NanoPortugal 2013.

13-15 February, 2013, Porto  - Portugal

Branquinho R, Veigas B, Pinto JV, Martins R, Baptista PV, and Fortunato E; 2011; Ta₂O₅ electrolyte-insulator-semiconductor sensor for Real Time PCR;

Bio-Sensing Technology 2011,

10-12 October, 2011, Amsterdam - The Netherlands.

Veigas, Bruno; Machado, Diana; Perdigão, João; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro; 2010. A PCR-Au-nanoprobes combined approach for detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis;

TNT2010-Congress

September 2010, Braga - Portugal.

Veigas, Bruno; Perdigão, João; Portugal, Isabel; Couto, Isabel; Viveiros, Miguel; Baptista, Pedro; Use of Au-nanoprobes for the detection of SNPs associated with antibiotic resistance in Mycobacterium tuberculosis;

MicroBiotec 2009-Congress,

November 2009, Vilamoura – Portugal

Prize: Best Poster in Nanotechnology

Patents (2)

Inventor - Integração de um método colorimétrico de deteção de ácidos nucleicos baseado em nanossondas num sistema de microfluídica para utilização portátil em ponto de interesse. March 2016. (Approved - July 2019)

Provisional Patent Application (1)

Inventor - Integração de um sensor flexível de efeito de campo modelado por carga para a deteção de polimerização de ácidos nucleicos. March 2016.

Participation in Research Projects

Inn-INDIGO/0002/2015        Project RADetect - One Platform - Multiple biomarker detection of Rheumatoid Arthritis.

PTDC/BTM-SAL/31201/2017 Integrated microfluidic device for cancer detection from free circulating DNA using Digital PCR.

Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/BBB-NAN/1812/2012   Silence is golden (siAu) - silencing the silencers via   multifunctional gold nanoconjugates towards cancer therapy.

Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/QUI-QUI/112597/2009            NANOLIGHT - Nanosystems for delivery of caged compounds

                                               Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/CVT/111634/2009       zoonTB - Improved NAT-based approaches to detect and discriminate animal-associated Mycobacterium tuberculosis Complex members and evaluation of the zoonotic potential of these species in Portugal

                                               Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/SAU-BEB/098125/2009 Portable nano/micro-biosensor array platform for ultrasensitive multidetection in blood samples ;

                                               Elvira Fortunato;

                                               Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/BIA-MIC/71280/2006 Mutational and physiological dynamics of drug resistance in Mycobacterium tuberculosis: the emergence of Multi-Drug Resistant Tuberculosis;

                                               Miguel Viveiros Bettencourt;

                                               Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/SAU-BEB/66511/2006 Nanodiagnostics: gold-nanoprobes for molecular recognition - application to DNA adducts detection; pathogen detection; gene expression and RNA quantification;

                                               Pedro Miguel Ribeiro Viana Baptista;

                                               Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

PTDC/BIO/66514/2006         Sensitive and selective detection of DNA/RNA based on functionalized gold nanoparticles - application to pathogen detection; mutation detection and RNA quantification;

                                               Pedro  Miguel Ribeiro Viana Baptista;

                                               Fundação da Faculdade de Ciências e Tecnologia (FFCT/FCT/UNL)

Present research interests

Molecular biology, diagnostic methods, biotechnology, nanotechnology, nobel metal nanoparticles, bionanotechnology, c, nanoPOC instrumentation and development for commercial purposes, project management, biosensors, field effect devices, ISFETs, BioFEDs.