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Research Output at NOVA 1997 2020

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2020
2 Citations (Scopus)

SLMP53-1 interacts with wild-type and mutant p53 DNA-binding domain and reactivates multiple hotspot mutations

Gomes, A. S., Ramos, H., Gomes, S., Loureiro, J. B., Soares, J., Barcherini, V., Monti, P., Fronza, G., Oliveira, C., Domingues, L., Bastos, M., Dourado, D. F. A. R., Carvalho, A. L., Romão, M. J., Pinheiro, B., Marcelo, F., Carvalho, A., Santos, M. M. M. & Saraiva, L., 1 Jan 2020, In : Biochimica et Biophysica Acta - General Subjects. 1864, 1, 129440.

Research output: Contribution to journalArticle

Open Access
Tumors
Mutation
Oncology
DNA
Ports and harbors
2019
Glucans
Carbohydrates
Ligands
Proteins
Chain length
2018
1 Citation (Scopus)
8 Downloads (Pure)

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Bule, P., Pires, V. M. R., Alves, V. D., Carvalho, A. L., Prates, J. A. M., Ferreira, L. M. A., Smith, S. P., Gilbert, H. J., Noach, I., Bayer, E. A., Najmudin, S. & Fontes, C. M. G. A., 1 Dec 2018, In : Scientific Reports. 8, 1, 6987.

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Ruminococcus
Rumen
Cellulose
Polysaccharides
3 Citations (Scopus)

Molecular Recognition of a Thomsen–Friedenreich Antigen Mimetic Targeting Human Galectin-3

Santarsia, S., Grosso, A. S., Trovão, F., Jiménez-Barbero, J., Carvalho, A. L., Nativi, C. & Marcelo, F., 8 Oct 2018, In : Chemmedchem. 13, 19, p. 2030-2036 7 p.

Research output: Contribution to journalArticle

Galectin 3
Molecular recognition
Antigens
Neoplasm Metastasis
Neoplasms
2 Citations (Scopus)

Novel insights into the degradation of β-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase

Kumar, K., Correia, M. A. S., Pires, V. M. R., Dhillon, A., Sharma, K., Rajulapati, V., Fontes, C. M. G. A., Carvalho, A. L. & Goyal, A., 1 Oct 2018, In : International Journal of Biological Macromolecules. 117, p. 890-901 12 p.

Research output: Contribution to journalArticle

Open Access
Cellulosomes
Clostridium thermocellum
Clostridium
Glucans
Glycoside Hydrolases
3 Citations (Scopus)
6 Downloads (Pure)
Open Access
File
Cytotoxicity
cultured cells
deoxyribonucleic acid
Cells
evaluation
2 Citations (Scopus)

Target highlights from the first post-PSI CASP experiment (CASP12, May–August 2016)

Kryshtafovych, A., Albrecht, R., Baslé, A., Bule, P., Caputo, A. T., Carvalho, A. L., Chao, K. L., Diskin, R., Fidelis, K., Fontes, C. M. G. A., Fredslund, F., Gilbert, H. J., Goulding, C. W., Hartmann, M. D., Hayes, C. S., Herzberg, O., Hill, J. C., Joachimiak, A., Kohring, G. W., Koning, R. I. & 19 others, Lo Leggio, L., Mangiagalli, M., Michalska, K., Moult, J., Najmudin, S., Nardini, M., Nardone, V., Ndeh, D., Nguyen, T. H., Pintacuda, G., Postel, S., van Raaij, M. J., Roversi, P., Shimon, A., Singh, A. K., Sundberg, E. J., Tars, K., Zitzmann, N. & Schwede, T., 1 Mar 2018, In : Proteins: Structure, Function and Bioinformatics. 86, p. 27-50 24 p.

Research output: Contribution to journalArticle

Proteins
Experiments
4 Citations (Scopus)
7 Downloads (Pure)

The crystal structure of the R280K mutant of human p53 explains the loss of DNA binding

Gomes, A. S., Trovão, F., Andrade Pinheiro, B., Freire, F., Gomes, S., Oliveira, C., Domingues, L., Romão, M. J., Saraiva, L. & Carvalho, A. L., 13 Apr 2018, In : International Journal of Molecular Sciences. 19, 4, 1184.

Research output: Contribution to journalArticle

Open Access
File
DNA
deoxyribonucleic acid
Crystal structure
crystal structure
cancer
2017
9 Citations (Scopus)

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

Bule, P., Alves, V. D., Israeli-Ruimy, V., Carvalho, A. L., Ferreira, L. M. A., Smith, S. P., Gilbert, H. J., Najmudin, S., Bayer, E. A. & Fontes, C. M. G. A., 7 Apr 2017, In : Scientific Reports. 7, 1, 759.

Research output: Contribution to journalArticle

Cellulosomes
Ruminococcus
cohesins
Enzymes
Carbohydrates
4 Citations (Scopus)
Crystallization
Self assembly
self assembly
crystallization
Infrared radiation
5 Citations (Scopus)

Ion-Exchange Membranes for Stable Derivatization of Protein Crystals

Polino, M., Carvalho, A. L., Juknaite, L., Portugal, C. A. M., Coelhoso, I. M., Romão, M. J. & Crespo, J. G., 6 Sep 2017, In : Crystal Growth and Design. 17, 9, p. 4563-4572 10 p.

Research output: Contribution to journalArticle

Ion exchange membranes
membranes
proteins
Proteins
Crystals
3 Citations (Scopus)

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C

Pires, V. M. R., Pereira, P. M., Brás, J. L. A., Correia, M., Cardoso, V., Bule, P., Alves, V. D., Najmudin, S., Venditto, I., Ferreira, L. M. A., Romão, M. J., Carvalho, A. L., Fontes, C. M. G. A. & Prazeres, D. M., 24 Mar 2017, In : Journal of Biological Chemistry. 292, 12, p. 4847-4860 14 p.

Research output: Contribution to journalArticle

Spirochaeta
Carbohydrates
Ligands
Cellulose
Plant Cells
20 Citations (Scopus)

Tunable Gas Sensing Gels by Cooperative Assembly

Hussain, A., Semeano, A. T. S., Palma, S. I. C. J., Pina, A. S., Almeida, J., Medrado, B. F., Pádua, A. C. C. S., Carvalho, A. L., Dionísio, M., Li, R. W. C., Gamboa, H., Ulijn, R. V., Gruber, J. & Roque, A. C. A., 19 Jul 2017, In : Advanced Functional Materials. 27, 27, 1700803.

Research output: Contribution to journalArticle

Gels
assembly
Gases
gels
gases
2016
10 Citations (Scopus)
2 Downloads (Pure)

Diverse specificity of cellulosome attachment to the bacterial cell surface

Brás, J. L. A., Pinheiro, B. A., Cameron, K., Cuskin, F., Viegas, A., Najmudin, S., Bule, P., Pires, V. M. R., Romão, M. J., Bayer, E. A., Spencer, H. L., Smith, S. P., Gilbert, H. J., Alves, V. D., Carvalho, A. L. & Fontes, C. M. G. A., 7 Dec 2016, In : Scientific Reports. 6, p. 38292

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Anaerobic Bacteria
Proteins
Carbohydrates
cohesins
2015
5 Citations (Scopus)

Ionic-Liquid-Functionalized Mineral Particles for Protein Crystallization

Kowacz, M., Marchel, M., Juknaité, L., Esperança, J. M. S. S., Romão, M. J., Carvalho, A. L. & Rebelo, L. P. N., Jun 2015, In : Crystal Growth & Design. 15, 6, p. 2994-3003 10 p.

Research output: Contribution to journalArticle

Ionic Liquids
Crystallization
Ionic liquids
Minerals
minerals
31 Citations (Scopus)

Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry

Palma, M. A. D. S., Liu, Y., Zhang, H., Zhang, Y., McCleary, B. V., Yu, G., Huang, Q., Guidolin, L. S., Ciocchini, A. E., Torosantucci, A., Wang, D., Carvalho, A. L., Fontes, C. M. G. A., Mulloy, B., Childs, R. A., Feizi, T. & Chai, W., 1 Apr 2015, In : Molecular & Cellular Proteomics. 14, 4, p. 974-988 15 p.

Research output: Contribution to journalArticle

Glucans
Microarrays
Mass spectrometry
Mass Spectrometry
Oligosaccharides
2013
13 Citations (Scopus)
Clostridium thermocellum
Carbohydrates
Ligands
Entropy
Proteins
2012
4 Citations (Scopus)

Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes

Brás, J. L. A., Carvalho, A. L., Viegas, A., Najmudin, S., Alves, V. D., Prates, J. A. M., Ferreira, L. M. A., Romão, M. J., Gilbert, H. J. & Fontes, C. M. G. A., 22 May 2012, In : Methods in Enzymology. 510, p. 395-415 21 p.

Research output: Contribution to journalArticle

Cellulosomes
Bacterial Structures
Crystallization
Escherichia coli
Purification
27 Citations (Scopus)
Open Access
Ionic Liquids
Crystallization
Ionic liquids
crystallization
proteins
20 Citations (Scopus)

Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode

Brás, J. L. A., Alves, V. D., Carvalho, A. L., Najmudin, S., Prates, J. A. M., Ferreira, L. M. A., Bolam, D. N., Romão, M. J., Gilbert, H. J. & Fontes, C. M. G. A., 28 Dec 2012, In : Journal of Biological Chemistry. 287, 53, p. 44394-44405 12 p.

Research output: Contribution to journalArticle

Open Access
File
Clostridium thermocellum
Clostridium
Cellulosomes
Biological Phenomena
Cellulase
2011
24 Citations (Scopus)
Open Access
61 Citations (Scopus)
Open Access
2009
13 Citations (Scopus)

Liquid crystalline cellulose derivative elastomer films under uniaxial strain

DCM Group Author, Carvalho, A. L. M. D. & Godinho, M. H. F., 1 Jan 2009, In : Cellulose. 16, 2, p. 199-205

Research output: Contribution to journalArticle

Open Access
2 Citations (Scopus)
2 Downloads (Pure)
Open Access
File
Heme
Carrier Proteins
proteins
Crystallization
Purification
17 Citations (Scopus)

Real-Time Monitoring of Molecular Dynamics of Ethylene Glycol Dimethacrylate Glass Former

Carvalho, A. L. M. D., Correia, N. D. F. T., Romão, M. J. & DQ Group Author, 1 Jan 2009, In : Journal Of Physical Chemistry B. 113, 43, p. 14209-14217

Research output: Contribution to journalArticle

2008
25 Citations (Scopus)

Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - An NMR, X-ray crystallography and computational chemistry approach

Viegas, A., Brás, N. F., Cerqueira, N. M. F. S. A., Fernandes, P. A., Prates, J. A. M., Fontes, C. M. G. A., Bruix, M., Romão, M. J., Carvalho, A. L., Ramos, M. J., Macedo, A. L. & Cabrita, E. J., 1 May 2008, In : FEBS Journal. 275, 10, p. 2524-2535 12 p.

Research output: Contribution to journalArticle

Computational chemistry
X ray crystallography
X Ray Crystallography
Clostridium thermocellum
Cellobiose
2007
100 Citations (Scopus)
Open Access
2006
35 Citations (Scopus)
Treponema pallidum
Iron
Crystal structure
Ligands
Superoxides
82 Citations (Scopus)
2 Downloads (Pure)

Xyloglucan is recognized by carbohydrate-binding modules that interact with β-glucan chains

Najmudin, S., Guerreiro, C. I. P. D., Carvalho, A. L., Prates, J. A. M., Correia, M. A. S., Alves, V. D., Ferreira, L. M. A., Romão, M. J., Gilbert, H. J., Bolam, D. N. & Fontes, C. M. G. A., 31 Mar 2006, In : Journal of Biological Chemistry. 281, 13, p. 8815-8828 14 p.

Research output: Contribution to journalArticle

Open Access
File
Glucans
Carbohydrates
Cellulose
Plant Cells
Ligands
2005
31 Citations (Scopus)

Insights into the structural determinants of cohesin - Dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA

Carvalho, A. L., Pires, V. M. R., Gloster, T. M., Turkenburg, J. P., Prates, J. A. M., Ferreira, L. M. A., Romão, M. J., Davies, G. J., Fontes, C. M. G. A. & Gilbert, H. J., 24 Jun 2005, In : Journal of Molecular Biology. 349, 5, p. 909-915 7 p.

Research output: Contribution to journalArticle

Clostridium thermocellum
Cellulosomes
Binding Sites
Multienzyme Complexes
Plant Cells
5 Citations (Scopus)
2 Downloads (Pure)
Open Access
File
syphilis
Treponema pallidum
Spirochaetales
inorganic peroxides
Syphilis
2004
83 Citations (Scopus)
1 Downloads (Pure)

The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates β-1,4- and β-1,3-1,4-mixed linked glucans at a single binding site

Carvalho, A. L., Goyal, A., Prates, J. A. M., Bolam, D. N., Gilbert, H. J., Pires, V. M. R., Ferreira, L. M. A., Planas, A., Romão, M. J. & Fontes, C. M. G. A., 13 Aug 2004, In : Journal of Biological Chemistry. 279, 33, p. 34785-34793 9 p.

Research output: Contribution to journalArticle

Open Access
File
Clostridium thermocellum
Clostridium
Glucans
Binding Sites
Carbohydrates
2003
194 Citations (Scopus)

Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex

Carvalho, A. L., Dias, F. M. V., Prates, J. A. M., Nagy, T., Gilbert, H. J., Davies, G. J., Ferreira, L. M. A., Romão, M. J. & Fontes, C. M. G. A., 25 Nov 2003, In : Proceedings of the National Academy of Sciences of the United States of America. 100, SUPPL. 2, p. 13809-13814 6 p.

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Helix-Loop-Helix Motifs
Clostridium thermocellum
EF Hand Motifs
Multiprotein Complexes
2002
69 Citations (Scopus)

Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA

Carvalho, A. L., Sanz, L., Barettino, D., Romero, A., Calvete, J. J. & Romão, M. J., 1 Jan 2002, In : Journal of Molecular Biology. 322, 2, p. 325-337 13 p.

Research output: Contribution to journalArticle

Kallikreins
Semen
Prostate
Horses
Serine Proteases
2001
2 Citations (Scopus)
Kallikreins
Crystallization
Semen
X-Ray Diffraction
Horses
1999
17 Citations (Scopus)
3 Downloads (Pure)
Open Access
File
Rubredoxins
X-Ray Diffraction
Metals
X ray diffraction
Proteins
1997
6 Citations (Scopus)

Crystallization and preliminary X-ray diffraction studies of aSFP, a bovine seminal plasma protein with a single CUB domain architecture

Dias, J. M. L., Carvalho, A. L., Kölln, I., Calvete, J. J., Töpfer-Petersen, E., Varela, P. F., Romero, A., Urbanke, C. & Romão, M. J., 1997, In : Protein Science. 6, 3, p. 725-727 3 p.

Research output: Contribution to journalArticle

Seminal Plasma Proteins
Crystallization
X-Ray Diffraction
X ray diffraction
Fluids
41 Citations (Scopus)

Crystal structure of acidic seminal fluid protein (aSFP) at 1.9 Å resolution: a bovine polypeptide of the spermadhesin family

Romão, M. J., Kölln, I., Dias, J. M., Carvalho, A. L., Romero, A., Varela, P. F., Sanz, L., Töpfer-Petersen, E. & Calvete, J. J., 12 Dec 1997, In : Journal of Molecular Biology. 274, 4, p. 650-660 11 p.

Research output: Contribution to journalArticle

Peptides
Proteins
R388
spermadhesin
Amino Acids
124 Citations (Scopus)

The crystal structures of two spermadhesins reveal the CUB domain fold

Romero, A., Romão, M. J., Varela, P. F., Kölln, I., Dias, J. M., Carvalho, A. L., Sanz, L., Töpfer-Petersen, E. & Calvete, J. J., 1 Jan 1997, In : Nature Structural Biology. 4, 10, p. 783-788 6 p.

Research output: Contribution to journalArticle

Crystal structure
Semen
Lectins
Ovum
Spermatozoa