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Research Output at NOVA 1997 2020

2020
2 Citations (Scopus)

SLMP53-1 interacts with wild-type and mutant p53 DNA-binding domain and reactivates multiple hotspot mutations

Gomes, A. S., Ramos, H., Gomes, S., Loureiro, J. B., Soares, J., Barcherini, V., Monti, P., Fronza, G., Oliveira, C., Domingues, L., Bastos, M., Dourado, D. F. A. R., Carvalho, A. L., Romão, M. J., Pinheiro, B., Marcelo, F., Carvalho, A., Santos, M. M. M. & Saraiva, L., 1 Jan 2020, In : Biochimica et Biophysica Acta - General Subjects. 1864, 1, 129440.

Research output: Contribution to journalArticle

Open Access
Tumors
Mutation
Oncology
DNA
Ports and harbors
2019
Glucans
Carbohydrates
Ligands
Proteins
Chain length
2018
1 Citation (Scopus)
8 Downloads (Pure)

Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Bule, P., Pires, V. M. R., Alves, V. D., Carvalho, A. L., Prates, J. A. M., Ferreira, L. M. A., Smith, S. P., Gilbert, H. J., Noach, I., Bayer, E. A., Najmudin, S. & Fontes, C. M. G. A., 1 Dec 2018, In : Scientific Reports. 8, 1, 6987.

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Ruminococcus
Rumen
Cellulose
Polysaccharides
3 Citations (Scopus)

Molecular Recognition of a Thomsen–Friedenreich Antigen Mimetic Targeting Human Galectin-3

Santarsia, S., Grosso, A. S., Trovão, F., Jiménez-Barbero, J., Carvalho, A. L., Nativi, C. & Marcelo, F., 8 Oct 2018, In : Chemmedchem. 13, 19, p. 2030-2036 7 p.

Research output: Contribution to journalArticle

Galectin 3
Molecular recognition
Antigens
Neoplasm Metastasis
Neoplasms
2 Citations (Scopus)

Novel insights into the degradation of β-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase

Kumar, K., Correia, M. A. S., Pires, V. M. R., Dhillon, A., Sharma, K., Rajulapati, V., Fontes, C. M. G. A., Carvalho, A. L. & Goyal, A., 1 Oct 2018, In : International Journal of Biological Macromolecules. 117, p. 890-901 12 p.

Research output: Contribution to journalArticle

Open Access
Cellulosomes
Clostridium thermocellum
Clostridium
Glucans
Glycoside Hydrolases

Structural elucidation of macromolecules

Romão, M. J., Santos-Silva, T. S., Carvalho, A. L., Cabrita, E. J. & Marcelo, F. M. B. M., 2018, Essential Techniques for Medical and Life Scientists : A Guide to Contemporary Methods and Current Applications With the Protocols. Bentham Science Publishers Ltd., Vol. Pt.1. p. 30-91 61 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

Macromolecules
Ligands
X ray crystallography
X ray scattering
Nucleic Acids
3 Citations (Scopus)
6 Downloads (Pure)
Open Access
File
Cytotoxicity
cultured cells
deoxyribonucleic acid
Cells
evaluation
2 Citations (Scopus)

Target highlights from the first post-PSI CASP experiment (CASP12, May–August 2016)

Kryshtafovych, A., Albrecht, R., Baslé, A., Bule, P., Caputo, A. T., Carvalho, A. L., Chao, K. L., Diskin, R., Fidelis, K., Fontes, C. M. G. A., Fredslund, F., Gilbert, H. J., Goulding, C. W., Hartmann, M. D., Hayes, C. S., Herzberg, O., Hill, J. C., Joachimiak, A., Kohring, G. W., Koning, R. I. & 19 others, Lo Leggio, L., Mangiagalli, M., Michalska, K., Moult, J., Najmudin, S., Nardini, M., Nardone, V., Ndeh, D., Nguyen, T. H., Pintacuda, G., Postel, S., van Raaij, M. J., Roversi, P., Shimon, A., Singh, A. K., Sundberg, E. J., Tars, K., Zitzmann, N. & Schwede, T., 1 Mar 2018, In : Proteins: Structure, Function and Bioinformatics. 86, p. 27-50 24 p.

Research output: Contribution to journalArticle

Proteins
Experiments

Targeting protein-carbohydrate interactions in plant cell-wall biodegradation: the power of carbohydrate microarrays

Ribeiro, D. O., Pinheiro, B. A., Carvalho, A. L. & Palma, A. S., 2018, Carbohydrate Chemistry: Chemical and Biological Approaches Volume 43. The Royal Society of Chemistry, Vol. 43. p. 159-176 18 p.

Research output: Chapter in Book/Report/Conference proceedingChapter

targeting
carbohydrate
biodegradation
protein
polysaccharide
4 Citations (Scopus)
7 Downloads (Pure)

The crystal structure of the R280K mutant of human p53 explains the loss of DNA binding

Gomes, A. S., Trovão, F., Andrade Pinheiro, B., Freire, F., Gomes, S., Oliveira, C., Domingues, L., Romão, M. J., Saraiva, L. & Carvalho, A. L., 13 Apr 2018, In : International Journal of Molecular Sciences. 19, 4, 1184.

Research output: Contribution to journalArticle

Open Access
File
DNA
deoxyribonucleic acid
Crystal structure
crystal structure
cancer
2017
9 Citations (Scopus)

Assembly of Ruminococcus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes

Bule, P., Alves, V. D., Israeli-Ruimy, V., Carvalho, A. L., Ferreira, L. M. A., Smith, S. P., Gilbert, H. J., Najmudin, S., Bayer, E. A. & Fontes, C. M. G. A., 7 Apr 2017, In : Scientific Reports. 7, 1, 759.

Research output: Contribution to journalArticle

Cellulosomes
Ruminococcus
cohesins
Enzymes
Carbohydrates
4 Citations (Scopus)
Crystallization
Self assembly
self assembly
crystallization
Infrared radiation
5 Citations (Scopus)

Ion-Exchange Membranes for Stable Derivatization of Protein Crystals

Polino, M., Carvalho, A. L., Juknaite, L., Portugal, C. A. M., Coelhoso, I. M., Romão, M. J. & Crespo, J. G., 6 Sep 2017, In : Crystal Growth and Design. 17, 9, p. 4563-4572 10 p.

Research output: Contribution to journalArticle

Ion exchange membranes
membranes
proteins
Proteins
Crystals
23 Citations (Scopus)

Renaissance of protein crystallization and precipitation in biopharmaceuticals purification

Dos Santos, R., Roque, A. C. A. & Carvalho, A. L., 1 Jan 2017, In : Biotechnology Advances. 35, 1, p. 41-50 10 p.

Research output: Contribution to journalReview article

Crystallization
Purification
Proteins
Pipelines
Molecules
3 Citations (Scopus)

Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C

Pires, V. M. R., Pereira, P. M., Brás, J. L. A., Correia, M., Cardoso, V., Bule, P., Alves, V. D., Najmudin, S., Venditto, I., Ferreira, L. M. A., Romão, M. J., Carvalho, A. L., Fontes, C. M. G. A. & Prazeres, D. M., 24 Mar 2017, In : Journal of Biological Chemistry. 292, 12, p. 4847-4860 14 p.

Research output: Contribution to journalArticle

Spirochaeta
Carbohydrates
Ligands
Cellulose
Plant Cells
20 Citations (Scopus)

Tunable Gas Sensing Gels by Cooperative Assembly

Hussain, A., Semeano, A. T. S., Palma, S. I. C. J., Pina, A. S., Almeida, J., Medrado, B. F., Pádua, A. C. C. S., Carvalho, A. L., Dionísio, M., Li, R. W. C., Gamboa, H., Ulijn, R. V., Gruber, J. & Roque, A. C. A., 19 Jul 2017, In : Advanced Functional Materials. 27, 27, 1700803.

Research output: Contribution to journalArticle

Gels
assembly
Gases
gels
gases
2016

An integrative strategy to decipher glycan recognition in the human gut microbiome

Correia, V. G., Bras, J. L. A., Liu, Y., Silva, L., Zhang, Y., Pinheiro, B. A., Romao, M. J., Carvalho, A. L., Chai, W., Fontes, C. M. G. A., Feizi, T. & Palma, A. S., Dec 2016, In : Glycobiology. 26, 12, p. 1398-1399 2 p.

Research output: Contribution to journalMeeting Abstract

10 Citations (Scopus)
2 Downloads (Pure)

Diverse specificity of cellulosome attachment to the bacterial cell surface

Brás, J. L. A., Pinheiro, B. A., Cameron, K., Cuskin, F., Viegas, A., Najmudin, S., Bule, P., Pires, V. M. R., Romão, M. J., Bayer, E. A., Spencer, H. L., Smith, S. P., Gilbert, H. J., Alves, V. D., Carvalho, A. L. & Fontes, C. M. G. A., 7 Dec 2016, In : Scientific Reports. 6, p. 38292

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Anaerobic Bacteria
Proteins
Carbohydrates
cohesins
2015
5 Citations (Scopus)

Ionic-Liquid-Functionalized Mineral Particles for Protein Crystallization

Kowacz, M., Marchel, M., Juknaité, L., Esperança, J. M. S. S., Romão, M. J., Carvalho, A. L. & Rebelo, L. P. N., Jun 2015, In : Crystal Growth & Design. 15, 6, p. 2994-3003 10 p.

Research output: Contribution to journalArticle

Ionic Liquids
Crystallization
Ionic liquids
Minerals
minerals
31 Citations (Scopus)

Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry

Palma, M. A. D. S., Liu, Y., Zhang, H., Zhang, Y., McCleary, B. V., Yu, G., Huang, Q., Guidolin, L. S., Ciocchini, A. E., Torosantucci, A., Wang, D., Carvalho, A. L., Fontes, C. M. G. A., Mulloy, B., Childs, R. A., Feizi, T. & Chai, W., 1 Apr 2015, In : Molecular & Cellular Proteomics. 14, 4, p. 974-988 15 p.

Research output: Contribution to journalArticle

Glucans
Microarrays
Mass spectrometry
Mass Spectrometry
Oligosaccharides
2014

Erratum: Use of gold nanoparticles as additives in protein crystallization (Crystal Growth and Design (2014) 14:1 (222-227) DOI:10.1021/cg4014398)

Ribeiro, D., Kulakova, A., Quaresma, P., Pereira, E., Bonifácio, C., Romão, M. J., Franco, R. & Carvalho, A. L., 5 Feb 2014, In : Crystal Growth & Design. 14, 2, p. 888 1 p.

Research output: Contribution to journalComment/debate

Open Access
2013

Determinants of selective group reduction in the TNT-bound xenobiotic reductase B from P. putida

Carvalho, A. L. M. D. & Romão, M. J., 1 Jan 2013, 233 SPRING ST, NEW YORK, NY 10013 USA : Springer.

Research output: Other contribution

13 Citations (Scopus)
Clostridium thermocellum
Carbohydrates
Ligands
Entropy
Proteins
2012

Designer-oligosaccharide microarrays to decipher ligands in mammalian and prokaryotic glucan-recognition systems

Palma, A. S., Liu, Y., Zhang, Y., Zhang, H., Luis, A. S., Carvalho, A. L., Gilbert, H. J., Boraston, A., Fontes, C. M. G. A., Chai, W. & Ten Feizi, Nov 2012, In : Glycobiology. 22, 11, p. 1612-1613

Research output: Contribution to journalMeeting Abstract

4 Citations (Scopus)

Escherichia coli expression, purification, crystallization, and structure determination of bacterial cohesin-dockerin complexes

Brás, J. L. A., Carvalho, A. L., Viegas, A., Najmudin, S., Alves, V. D., Prates, J. A. M., Ferreira, L. M. A., Romão, M. J., Gilbert, H. J. & Fontes, C. M. G. A., 22 May 2012, In : Methods in Enzymology. 510, p. 395-415 21 p.

Research output: Contribution to journalArticle

Cellulosomes
Bacterial Structures
Crystallization
Escherichia coli
Purification
4 Citations (Scopus)

Flexibility and specificity of the cohesindockerin interaction: Implications for cellulosome assembly and functionality

Pinheiro, B. A., Brás, J. L. A., Najmudin, S., Carvalho, A. L., Ferreira, L. M. A., Prates, J. A. M. & Fontes, C. M. G. A., 1 May 2012, In : Biocatalysis And Biotransformation. 30, 3, p. 309-315 7 p.

Research output: Contribution to journalReview article

Cellulosomes
Enzymes
Carbohydrates
Scaffolds
Cellulose
27 Citations (Scopus)
Open Access
Ionic Liquids
Crystallization
Ionic liquids
crystallization
proteins
20 Citations (Scopus)

Novel Clostridium thermocellum type I cohesin-dockerin complexes reveal a single binding mode

Brás, J. L. A., Alves, V. D., Carvalho, A. L., Najmudin, S., Prates, J. A. M., Ferreira, L. M. A., Bolam, D. N., Romão, M. J., Gilbert, H. J. & Fontes, C. M. G. A., 28 Dec 2012, In : Journal of Biological Chemistry. 287, 53, p. 44394-44405 12 p.

Research output: Contribution to journalArticle

Open Access
File
Clostridium thermocellum
Clostridium
Cellulosomes
Biological Phenomena
Cellulase
2011
24 Citations (Scopus)
Open Access
61 Citations (Scopus)
Open Access
1 Citation (Scopus)

Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis (Proceedings of the National Academy of Sciences of the United States of America (2011) 108, 13, (5237-5242))

Brás, J. L. A., Cartmell, A., Carvalho, A. L. M., Verzé, G., Bayer, E. A., Vazana, Y., Correia, M. A. S., Prates, J. A. M., Ratnaparkhe, S., Boraston, A. B., Romão, M. J., Fontes, C. M. G. A. & Gilbert, H. J., 17 May 2011, In : Proceedings of the National Academy of Sciences of the United States of America. 108, 20, p. 8525 1 p.

Research output: Contribution to journalComment/debate

Open Access
File
2009
13 Citations (Scopus)

Liquid crystalline cellulose derivative elastomer films under uniaxial strain

DCM Group Author, Carvalho, A. L. M. D. & Godinho, M. H. F., 1 Jan 2009, In : Cellulose. 16, 2, p. 199-205

Research output: Contribution to journalArticle

Open Access
2 Citations (Scopus)
2 Downloads (Pure)
Open Access
File
Heme
Carrier Proteins
proteins
Crystallization
Purification
17 Citations (Scopus)

Real-Time Monitoring of Molecular Dynamics of Ethylene Glycol Dimethacrylate Glass Former

Carvalho, A. L. M. D., Correia, N. D. F. T., Romão, M. J. & DQ Group Author, 1 Jan 2009, In : Journal Of Physical Chemistry B. 113, 43, p. 14209-14217

Research output: Contribution to journalArticle

22 Citations (Scopus)

X-ray crystallography in drug discovery

Carvalho, A. L., Trincão, J. & Romão, M. J., 1 Jan 2009, In : Methods In Molecular Biology (Clifton, N.J.). 572, p. 31-56 26 p.

Research output: Contribution to journalReview article

X Ray Crystallography
Drug Discovery
Ligands
Proteins
Drug Design
2008
25 Citations (Scopus)

Molecular determinants of ligand specificity in family 11 carbohydrate binding modules - An NMR, X-ray crystallography and computational chemistry approach

Viegas, A., Brás, N. F., Cerqueira, N. M. F. S. A., Fernandes, P. A., Prates, J. A. M., Fontes, C. M. G. A., Bruix, M., Romão, M. J., Carvalho, A. L., Ramos, M. J., Macedo, A. L. & Cabrita, E. J., 1 May 2008, In : FEBS Journal. 275, 10, p. 2524-2535 12 p.

Research output: Contribution to journalArticle

Computational chemistry
X ray crystallography
X Ray Crystallography
Clostridium thermocellum
Cellobiose
2007
100 Citations (Scopus)
Open Access

The human carnitine acylcarnitine translocase (hCACT): Strategies for its heterologous expression, purification and crystallization

Ventura, F. V., Violante, S., Gomes, C., Carvalho, A. L., Romão, M. J., Gaspar, M. M., Cruz, M. E. M., Soveral, G., Wanders, R. J., Leandro, P. & Tavares, V. D. A., Aug 2007, In : Journal of Inherited Metabolic Disease. 30, Suppl.1, p. 53-53 1 p.

Research output: Contribution to journalMeeting Abstract

2006
35 Citations (Scopus)
Treponema pallidum
Iron
Crystal structure
Ligands
Superoxides
82 Citations (Scopus)
2 Downloads (Pure)

Xyloglucan is recognized by carbohydrate-binding modules that interact with β-glucan chains

Najmudin, S., Guerreiro, C. I. P. D., Carvalho, A. L., Prates, J. A. M., Correia, M. A. S., Alves, V. D., Ferreira, L. M. A., Romão, M. J., Gilbert, H. J., Bolam, D. N. & Fontes, C. M. G. A., 31 Mar 2006, In : Journal of Biological Chemistry. 281, 13, p. 8815-8828 14 p.

Research output: Contribution to journalArticle

Open Access
File
Glucans
Carbohydrates
Cellulose
Plant Cells
Ligands
2005
31 Citations (Scopus)

Insights into the structural determinants of cohesin - Dockerin specificity revealed by the crystal structure of the type II cohesin from Clostridium thermocellum SdbA

Carvalho, A. L., Pires, V. M. R., Gloster, T. M., Turkenburg, J. P., Prates, J. A. M., Ferreira, L. M. A., Romão, M. J., Davies, G. J., Fontes, C. M. G. A. & Gilbert, H. J., 24 Jun 2005, In : Journal of Molecular Biology. 349, 5, p. 909-915 7 p.

Research output: Contribution to journalArticle

Clostridium thermocellum
Cellulosomes
Binding Sites
Multienzyme Complexes
Plant Cells
5 Citations (Scopus)
2 Downloads (Pure)
Open Access
File
syphilis
Treponema pallidum
Spirochaetales
inorganic peroxides
Syphilis
2004
83 Citations (Scopus)
1 Downloads (Pure)

The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates β-1,4- and β-1,3-1,4-mixed linked glucans at a single binding site

Carvalho, A. L., Goyal, A., Prates, J. A. M., Bolam, D. N., Gilbert, H. J., Pires, V. M. R., Ferreira, L. M. A., Planas, A., Romão, M. J. & Fontes, C. M. G. A., 13 Aug 2004, In : Journal of Biological Chemistry. 279, 33, p. 34785-34793 9 p.

Research output: Contribution to journalArticle

Open Access
File
Clostridium thermocellum
Clostridium
Glucans
Binding Sites
Carbohydrates
2003
194 Citations (Scopus)

Cellulosome assembly revealed by the crystal structure of the cohesin-dockerin complex

Carvalho, A. L., Dias, F. M. V., Prates, J. A. M., Nagy, T., Gilbert, H. J., Davies, G. J., Ferreira, L. M. A., Romão, M. J. & Fontes, C. M. G. A., 25 Nov 2003, In : Proceedings of the National Academy of Sciences of the United States of America. 100, SUPPL. 2, p. 13809-13814 6 p.

Research output: Contribution to journalArticle

Open Access
File
Cellulosomes
Helix-Loop-Helix Motifs
Clostridium thermocellum
EF Hand Motifs
Multiprotein Complexes