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Research Output at NOVA 2000 2019

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2019

Affinity-Triggered Assemblies Based on a Designed Peptide–Peptide Affinity Pair

Fernandes, C. S. M., Pina, A. S., Barbosa, A. J. M., Padrão, I., Duarte, F., Teixeira, C. A. S., Alves, V., Gomes, P., Fernandes, T. G., Dias, A. M. G. C. & Roque, A. C. A., 1 Nov 2019, In : Biotechnology Journal. 14, 11, 1800559.

Research output: Contribution to journalArticle

Hydrogels
Peptides
Proline
Culture Media
Stem Cells
1 Citation (Scopus)
Serum Albumin
Ligands
Triazines
Plasma (human)
Biomarkers
Open Access
Volatile Organic Compounds
Gelatin
Film thickness
Gels
Gases
12 Citations (Scopus)
38 Downloads (Pure)
Open Access
File
Anti-Bacterial Agents
Technology
Health Care Costs
Anti-Infective Agents
Microbiology
Peptidomimetics
Phosphoproteins
Cyclic Peptides
peptides
Peptides
2018
9 Citations (Scopus)
3 Downloads (Pure)
Open Access
File
Volatile Organic Compounds
Meta-Analysis
Smell
Information Storage and Retrieval
Artificial Intelligence
11 Citations (Scopus)

Tilapia fish microbial spoilage monitored by a single optical gas sensor

Semeano, A. T. S., Maffei, D. F., Palma, S., Li, R. W. C., Franco, B. D. G. M., Roque, A. C. A. & Gruber, J., 1 Jul 2018, In : Food Control. 89, p. 72-76 5 p.

Research output: Contribution to journalArticle

Tilapia
tilapia (common name)
spoilage
sensors (equipment)
Fishes
2017

Cover Image, Volume 114, Number 3, March 2017

Dias, A. M. G. C. & Roque, A. C. A., 1 Mar 2017, In : Biotechnology and Bioengineering. 114, 3, p. i

Research output: Contribution to journalArticle

Scaffolds (biology)
Scaffolds
Purification
Proteins
1 Citation (Scopus)

Magnetic fishing of recombinant green fluorescent proteins and tagged proteins with designed synthetic ligands

Fernandes, C. S. M., Pina, A. S., Batalha, Í. L. & Roque, A. C. A., 12 Dec 2017, In : Separation Science and Technology (Philadelphia). 52, 18, p. 2907-2915 9 p.

Research output: Contribution to journalArticle

Green Fluorescent Proteins
Ligands
Proteins
Bearings (structural)
Recovery
20 Citations (Scopus)

Tunable Gas Sensing Gels by Cooperative Assembly

Hussain, A., Semeano, A. T. S., Palma, S. I. C. J., Pina, A. S., Almeida, J., Medrado, B. F., Pádua, A. C. C. S., Carvalho, A. L., Dionísio, M., Li, R. W. C., Gamboa, H., Ulijn, R. V., Gruber, J. & Roque, A. C. A., 19 Jul 2017, In : Advanced Functional Materials. 27, 27, 1700803.

Research output: Contribution to journalArticle

Gels
assembly
Gases
gels
gases
2016
4 Citations (Scopus)

Affinity adsorbents for proline-rich peptide sequences: a new role for WW domains

Dias, A. M. G. C., Garcia, R. M. N. D. S., Iranzo, O. & Roque, A. C. A., 2016, In : RCS Advances. 6, 73, p. 68979-68988 10 p.

Research output: Contribution to journalArticle

11 Citations (Scopus)

Affitins for protein purification by affinity magnetic fishing

Fernandes, C. S. M., Garcia, R. M. N. D. S., Ottengy, S., Viecinski, A. C., Béhar, G., Mouratou, B., Pecorari, F. & Roque, A. C. A., 29 Jul 2016, In : Journal Of Chromatography A. 1457, p. 50-58 9 p.

Research output: Contribution to journalArticle

Muramidase
Proteins
Archaeal Proteins
Immunoglobulin G
Protein Engineering

An affinity triggered MRI nanoprobe for pH-dependent cell labeling

Palma, S. I. C. J., Fernandes, A. R. & Roque, A. C. A., 2016, In : RCS Advances. 6, 114, p. 113503-113512 10 p.

Research output: Contribution to journalArticle

Open Access
Nanoprobes
Labeling
Tumors
Succimer
Nanoparticles
6 Citations (Scopus)

Mimicking nature: Phosphopeptide enrichment using combinatorial libraries of affinity ligands

Batalha, I. L., Zhou, H., Lilley, K., Lowe, C. R. & Roque, A. C. A., 29 Jul 2016, In : Journal Of Chromatography A. 1457, p. 76-87 12 p.

Research output: Contribution to journalArticle

Phosphopeptides
Ligands
Peptides
Triazines
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
4 Citations (Scopus)
Ligands
Peptides
Phosphopeptides
Atomic Spectrophotometry
Biological Products
6 Citations (Scopus)

Retroviral particles are effectively purified on an affinity matrix containing peptides selected by phage-display

Fernandes, C. S. M., Barbosa, I., Castro, R., Pina, A. S. F. P. M., Coroadinha, A. S., Barbas, A. & Roque, A. C. A., 1 Dec 2016, In : Biotechnology Journal. 11, 12, p. 1513-1524 12 p.

Research output: Contribution to journalArticle

Open Access
Bacteriophages
Peptides
Retroviridae
Particle Size
Virion
5 Citations (Scopus)

Small synthetic ligands for the enrichment of viral particles pseudotyped with amphotropic murine leukemia virus envelope

Fernandes, C. S. M., Castro, R., Coroadinha, A. S. & Roque, A. C. A., 18 Mar 2016, In : Journal Of Chromatography A. 1438, p. 160-70 11 p.

Research output: Contribution to journalArticle

Murine Leukemia Viruses
Virion
Ligands
Triazines
Genome
9 Citations (Scopus)

The future of protein scaffolds as affinity reagents for purification

Dias, A. M. G. C. & Roque, A. C. A., 29 Aug 2016, In : Biotechnology and Bioengineering. 114, 3, p. 481-491

Research output: Contribution to journalArticle

Scaffolds (biology)
Scaffolds
Purification
Ligands
Proteins
3 Citations (Scopus)

Tryptophan tags and de novo designed complementary affinity ligands for the expression and purification of recombinant proteins

Pina, A. S., Carvalho, S., Dias, A. M. G. C., Guilherme, M., Pereira, M. A. S., Caraça, L. T., Coroadinha, A. S., Lowe, C. R. & Roque, A. C. A., 11 Nov 2016, In : Journal Of Chromatography A. 1472, p. 55-65 11 p.

Research output: Contribution to journalArticle

Open Access
Recombinant Proteins
Tryptophan
Purification
Ligands
Proteins
2015
2 Citations (Scopus)

An in silico and chemical approach towards small protein production and application in phosphoproteomics

Dias, A. M. G. C., Iranzo, O. & Roque, A. C. A., 2015, In : RCS Advances. 5, 25, p. 19743-19751 9 p.

Research output: Contribution to journalArticle

9 Citations (Scopus)

A value-added exopolysaccharide as a coating agent for MRI nanoprobes

Palma, S. I. C. J., Rodrigues, C. A. V., Carvalho, A., del Puerto Morales, M., Freitas, M. F. A. D., Fernandes, M. A. N. D. C. R., Cabral, J. M. S. & Roque, A. C. A., 14 Sep 2015, In : Nanoscale. 7, 34, p. 14272-14283 12 p.

Research output: Contribution to journalArticle

6 Citations (Scopus)

Biobased Monoliths for Adenovirus Purification

Fernandes, C. S. M., Gonçalves, B., Sousa, M., Martins, D. L., Barroso, T., Pina, A. S., Peixoto, C., Ricardo, A. I. N. M. A. D. O. & Roque, A. C. A., 1 Apr 2015, In : Acs Applied Materials & Interfaces. 7, 12, p. 6605-6612 8 p.

Research output: Contribution to journalArticle

Biopolymers
Purification
Environmental technology
Chitosan
Macromolecular Substances
3 Citations (Scopus)
4 Downloads (Pure)
Open Access
File
Metalloproteases
metalloproteinases
Proteins
proteins
botulinum toxin
12 Citations (Scopus)

Covalent coupling of gum arabic onto superparamagnetic iron oxide nanoparticles for MRI cell labeling: physicochemical and in vitro characterization

Palma, S. I. C. J., Carvalho, A., Cavaco-Silva, J., Martins, P., Marciello, M., Fernandes, M. A. N. D. C. R., del Puerto Morales, M. & Roque, A. C. A., 2015, In : Contrast media & molecular imaging. 10, 4, p. 320-328 9 p.

Research output: Contribution to journalArticle

36 Citations (Scopus)

Effects of phase transfer ligands on monodisperse iron oxide magnetic nanoparticles

Palma, S. I. C. J., Marciello, M., Carvalho, A., Veintemillas-Verdaguer, S., Morales, M. D. P. & Roque, A. C. A., 1 Jan 2015, In : Journal Of Colloid And Interface Science. 437, p. 147-55 9 p.

Research output: Contribution to journalArticle

Succimer
Nanoparticles
Ligands
Citric Acid
Hot Temperature
7 Citations (Scopus)

Mild and cost-effective green fluorescent protein purification employing small synthetic ligands

Pina, A. S., Dias, A. M. G. C., Ustok, F. I., El Khoury, G., Fernandes, C. S. M., Branco, R. J. F., Lowe, C. R. & Roque, A. C. A., 30 Oct 2015, In : Journal Of Chromatography A. 1418, p. 83-93 11 p.

Research output: Contribution to journalArticle

Green Fluorescent Proteins
Ligands
Costs and Cost Analysis
Hydrophobic and Hydrophilic Interactions
Immunoprecipitation
2 Citations (Scopus)

Purification of human antibodies from animal cell cultures using gum arabic coated magnetic particles

Alves, B. M., Borlido, L., Rosa, S. A. S. L., Silva, M. F., Aires-Barros, M. R., Roque, A. C. A. & Azevedo, A. M., May 2015, In : Journal of Chemical Technology and Biotechnology. 90, 5, p. 838-846 9 p.

Research output: Contribution to journalArticle

2014
15 Citations (Scopus)

An extracellular polymer at the interface of magnetic bioseparations

Dhadge, V. L., Morgado, P. I., Freitas, M. F. A. D., Reis, M. A., Azevedo, A. M., Aires-Barros, R. & Roque, A. C. A., 6 Nov 2014, In : Journal Of The Royal Society Interface. 11, 100, 20140743.

Research output: Contribution to journalArticle

Polysaccharides
Antibodies
Magnetic separation
Polymers
Immunoglobulin G
9 Citations (Scopus)

A tailor-made "tag-receptor" affinity pair for the purification of fusion proteins

Pina, A. S., Guilherme, M., Pereira, M. A. S., Fernandes, C. S. F. M., Branco, R. J. F., El Khoury, G., Lowe, C. R. & Roque, A. C. A., 7 Jul 2014, In : Chembiochem. 15, 10, p. 1423-1435 13 p.

Research output: Contribution to journalArticle

Purification
Fusion reactions
Green Fluorescent Proteins
Ligands
Proteins
5 Citations (Scopus)

A theoretical and experimental approach toward the development of affinity adsorbents for GFP and GFP-fusion proteins purification

Fernandes, C. S. M., Pina, A. S., Dias, A. M. G. C., Branco, R. J. F. & Roque, A. C. A., 30 Sep 2014, In : Journal of Biotechnology. 186, p. 13-20 8 p.

Research output: Contribution to journalArticle

Green Fluorescent Proteins
Adsorbents
Purification
Fusion reactions
Ligands
13 Citations (Scopus)

Exploring the potential of magnetic antimicrobial agents for water disinfection

Pina, A. S., Batalha, I. L., Fernandes, C. S. M., Aoki, M. A. & Roque, A. C. A., 1 Dec 2014, In : Water Research. 66, p. 160-168 9 p.

Research output: Contribution to journalArticle

Antimicrobial agents
Disinfection
disinfection
Polyethylene glycols
Nanoparticles
13 Citations (Scopus)

Hybrid Monoliths for Magnetically-Driven Protein Separations

Barroso, T., Casimiro, T., Ferraria, A. M., Mattioli, F., Ricardo, A. I. N. M. A. D. O. & Roque, A. C. A., 23 Jul 2014, In : Advanced Functional Materials. 24, 28, p. 4528-4541 14 p.

Research output: Contribution to journalArticle

16 Citations (Scopus)

Magnetic aqueous two phase fishing: a hybrid process technology for antibody purification

Dhadge, V. L., Rosa, S. A. S. L., Azevedo, A., Aires-Barros, R. & Roque, A. C. A., 25 Apr 2014, In : Journal Of Chromatography A. 1339, p. 59-64 6 p.

Research output: Contribution to journalArticle

Gum Arabic
Cell Culture Techniques
Dextrans
Technology
Antibodies
12 Citations (Scopus)
Open Access
Biomimetics
biomimetics
Staphylococcal Protein A
antibodies
purification
8 Citations (Scopus)

The interaction of polymer-coated magnetic nanoparticles with seawater

Kadar, E., Batalha, I. L., Fisher, A. & Roque, A. C. A., 15 Jul 2014, In : The Science of the total environment. 487, p. 771-777 7 p.

Research output: Contribution to journalArticle

Seawater
Polymers
polymer
Nanoparticles
seawater
2013
6 Citations (Scopus)
Open Access
Biomimetics
Immobilization
Immunoglobulin G
Ligands
Antibody Affinity
15 Citations (Scopus)

Boronic acid-modified magnetic materials for antibody purification

Dhadge, V. L., Hussain, A., Azevedo, A. M., Aires-Barros, R. & Roque, A. C. A., 2013, In : Journal Of The Royal Society Interface. 11, 91, 20130875.

Research output: Contribution to journalArticle

Boronic Acids
Magnetic materials
Antibodies
Purification
Immunoglobulin G
58 Citations (Scopus)

Challenges and opportunities in the purification of recombinant tagged proteins

Pina, A. S., Lowe, C. R. & Roque, A. C. A., 18 Dec 2013, In : Biotechnology Advances. 32, 2, p. 366-81 16 p.

Research output: Contribution to journalArticle

Recombinant proteins
Recombinant Proteins
Purification
Ligands
Proteins
17 Citations (Scopus)

Functional monolithic platforms: Chromatographic tools for antibody purification

Barroso, T., Hussain, A., Roque, A. C. A. & Ricardo, A. I. N. M. A. D. O., 2013, In : Biotechnology Journal. 8, 6(SI), p. 671-681

Research output: Contribution to journalArticle

Antibodies
Polymers
Monoclonal Antibodies
Research
Therapeutics
9 Citations (Scopus)

In situ magnetic separation of antibody fragments from Escherichia coli in complex media

Cerff, M., Scholz, A., Franzreb, M., Batalha, Í. L., Roque, A. C. A. & Posten, C., 2013, In : Bmc Biotechnology. 13, 44.

Research output: Contribution to journalArticle

Immunoglobulin Fragments
Triazines
Metals
Escherichia coli
Copper
114 Citations (Scopus)

Magnetic separations in biotechnology

Borlido, L., Azevedo, A. M., Roque, A. C. A. & Aires-Barros, M. R., 2013, In : Biotechnology Advances. 31, 8, p. 1374–1385

Research output: Contribution to journalArticle

Magnetic separation
Automation
Biotechnology
Adsorbents
Nucleic Acids
22 Citations (Scopus)

Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification

Borlido, L., Moura, L., Azevedo, A. M., Roque, A. C. A., Aires-Barros, M. R. & Farinha, J. P. S., 2013, In : Biotechnology Journal. 8, 6(SI), p. 709-717

Research output: Contribution to journalArticle

Nanoparticles
Monoclonal Antibodies
Dromaiidae
CHO Cells
Polymethyl Methacrylate
9 Citations (Scopus)
1 Downloads (Pure)

Bioinspired and Sustainable Chitosan Based Monoliths for Antibody Capture and Release

Barroso, T., Roque, A. C. A. & Ricardo, A. I. N. M. A. D. O., 2012, In : Rsc Advances. 2, 30, p. 11285-11294

Research output: Contribution to journalArticle

Open Access
File
Chitosan
Antibodies
Staphylococcal Protein A
Biomimetics
Cell culture
35 Citations (Scopus)
Art
Fluorescence
Staining and Labeling
Paint
Microscopy
20 Citations (Scopus)
13 Downloads (Pure)

Dextran-Coated Magnetic Supports Modified with a Biomimetic Ligand for IgG Purification

Santana, S. D. F., Dhadge, V. L. & Roque, A. C. A., 2012, In : Acs Applied Materials & Interfaces. 4, 11, p. 5907-5914

Research output: Contribution to journalArticle

Open Access
File
Dextran
Biomimetics
Dextrans
Purification
Immunoglobulin G
19 Citations (Scopus)

Fishing human monoclonal antibodies from a CHO cell supernatant with boronic acid magnetic particles

Borlido, L., Azevedo, A. M., Sousa, A. G., Oliveira, P. H., Roque, A. C. A. & Aires-Barros, M. R., 2012, In : Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 903, p. 163-170

Research output: Contribution to journalArticle

Boronic Acids
Cricetulus
Ovary
Monoclonal Antibodies
Staphylococcal Protein A
26 Citations (Scopus)

Fluorescence recognition of proteinaceous binders in works of art by a novel integrated system of investigation

Sandu, I. C. A., Roque, A. C. A., Matteini, P., Schaefer, S., Agati, G., Correia, C. R. & Viana, J. F. F. P., 2012, In : Microscopy Research And Technique. 75, 3, p. 316-324

Research output: Contribution to journalArticle

Paint
arts
Art
Binders
paints
11 Citations (Scopus)

Immobilization of enterokinase on magnetic supports for the cleavage of fusion proteins

Santana, S. D. F., Pina, A. S. & Roque, A. C. A., 2012, In : Journal of Biotechnology. 161, 3, p. 378-382

Research output: Contribution to journalArticle

Enteropeptidase
Immobilization
Fusion reactions
Proteins
Biopolymers
59 Citations (Scopus)

Platforms for enrichment of phosphorylated proteins and peptides in proteomics

Batalha, Í. L., Lowe, C. R. & Roque, A. C. A., 2012, In : Trends In Biotechnology. 30, 2, p. 100-110

Research output: Contribution to journalArticle

Proteomics
Peptides
Phosphorylation
Proteins
Mass spectrometry